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Stage-specific differences in the requirements for germline stem cell maintenance in the Drosophila ovary

Shcherbata, Halyna R. and Ward, Ellen J. and Fischer, Karin A. and Yu, Jenn-Yah and Reynolds, Steven H. and Chen, Chun-Hong and Xu, Peizhang and Hay, Bruce A. and Ruohola-Baker, Hannele (2007) Stage-specific differences in the requirements for germline stem cell maintenance in the Drosophila ovary. Cell Stem Cell, 1 (6). pp. 698-709. ISSN 1934-5909. PMCID PMC2211735. doi:10.1016/j.stem.2007.11.007. https://resolver.caltech.edu/CaltechAUTHORS:20170408-203956988

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Abstract

In this study, we uncover a role for microRNAs (miRNAs) in Drosophila germline stem cell (GSC) maintenance. Disruption of Dicer-1 function in GSCs during adult life results in GSC loss. Surprisingly, however, loss of Dicer-1 during development does not result in a GSC maintenance defect, although a defect is seen if both Dicer-1 and Dicer-2 function are disrupted. Loss of the bantam miRNA mimics the Dicer-1 maintenance defect when induced in adult GSCs, suggesting that bantam plays a key role in GSC self-renewal. Mad, a component of the TGF-β pathway, behaves similarly to Dicer-1: adult GSC maintenance requires Mad if it is lost during adult life, but not if it is lost during pupal development. Overall, these results show stage-specific differential sensitivity of GSC maintenance to certain perturbations and suggest that there may be a Dicer-2-dependent GSC maintenance mechanism during development that is lost in later life.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.stem.2007.11.007DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2211735PubMed CentralArticle
Additional Information:© 2007 Elsevier. Received 24 April 2007, Revised 11 September 2007, Accepted 21 November 2007, Available online 12 December 2007. We thank Drs. A. Spradling, D. Pan, B. Calvi, S. Cohen, T.T. Su, T. Orr-Weaver, T. Xie, J. Tamkun, and R. Carthew for suggestions, flies, and reagents, Christian Nelson, Louise von Stechow, and Andriy Yatsenko for help with experiments, and Dr. Bradford Stadler for help with QPCR analysis. This work was supported by AHA fellowships for H.R.S. and S.H.R. and MOD and NIH grants and the Tietze Fellowship for H.R-B.
Funders:
Funding AgencyGrant Number
American Heart AssociationUNSPECIFIED
Ministry of Defence (UK)UNSPECIFIED
NIHUNSPECIFIED
Tietze FellowshipUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:6
PubMed Central ID:PMC2211735
DOI:10.1016/j.stem.2007.11.007
Record Number:CaltechAUTHORS:20170408-203956988
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170408-203956988
Official Citation:Halyna R. Shcherbata, Ellen J. Ward, Karin A. Fischer, Jenn-Yah Yu, Steven H. Reynolds, Chun-Hong Chen, Peizhang Xu, Bruce A. Hay, Hannele Ruohola-Baker, Stage-Specific Differences in the Requirements for Germline Stem Cell Maintenance in the Drosophila Ovary, Cell Stem Cell, Volume 1, Issue 6, 2007, Pages 698-709, ISSN 1934-5909, https://doi.org/10.1016/j.stem.2007.11.007. (http://www.sciencedirect.com/science/article/pii/S1934590907002809)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:76390
Collection:CaltechAUTHORS
Deposited By: 1Science Import
Deposited On:30 Mar 2018 23:16
Last Modified:15 Nov 2021 16:59

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