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Activation of phospholipase C beta4 by heterotrimeric GTP-binding proteins

Jiang, Huiping and Wu, Dianqing and Simon, Melvin I. (1994) Activation of phospholipase C beta4 by heterotrimeric GTP-binding proteins. Journal of Biological Chemistry, 269 (10). pp. 7593-7596. ISSN 0021-9258.

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Transient transfection assays were used to determine how the activity of phospholipase C beta 4, which is preferentially expressed in retina, was regulated. An expression vector carrying the full-length cDNA corresponding to phospholipase C beta 4 was constructed and co- transfected into COS-7 cells together with cDNA encoding the alpha subunits of the Gq class and various beta and gamma subunits corresponding to the heterotrimeric GTP-binding proteins. We found that all the alpha subunits of the Gq class, including G alpha q, G alpha 11, G alpha 14, G alpha 15, and G alpha 16 could activate PLC beta 4 and that none of the G beta gamma subunits that we tested including G beta 1 gamma 1, G beta 1 gamma 2, G beta 1 gamma 3, or G beta 2 gamma 2 activated phospholipase C beta 4. In control experiments, cotransfection with cDNA encoding the alpha subunit of transducin or Gi2 gave no activation of PLC beta 4. These results indicate that phospholipase C beta 4 is activated by G alpha subunits that are members of the Gq class, and, like the phospholipase C beta 1 isoform, it is refractory to activation in the transfection assay by many of the combinations of beta and gamma subunits found in the heterotrimeric G- proteins.

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Additional Information:Copyright © 1994 by the American Society for Biochemistry and Molecular Biology. (Received for publication, October 8, 1993, and in revised form, November 30, 1993) We would like to thank Drs. W. Pak for the partial PLC β4 cDNA and C.-H. Lee for the PLC β4 antibodies.
Issue or Number:10
Record Number:CaltechAUTHORS:JIAjbc94
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7640
Deposited By: Archive Administrator
Deposited On:15 Mar 2007
Last Modified:02 Oct 2019 23:44

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