Maurer, Joshua A. and Elmore, Donald E. and Clayton, Daniel and Xiong, Li and Lester, Henry A. and Dougherty, Dennis A. (2008) Confirming the Revised C-Terminal Domain of the MscL Crystal Structure. Biophysical Journal, 94 (12). pp. 4662-4667. ISSN 0006-3495. PMCID PMC2397327. doi:10.1529/biophysj.107.127365. https://resolver.caltech.edu/CaltechAUTHORS:20170409-064941478
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20170409-064941478
Abstract
The structure of the C-terminal domain of the mechanosensitive channel of large conductance (MscL) has generated significant controversy. As a result, several structures have been proposed for this region: the original crystal structure (1MSL) of the Mycobacterium tuberculosis homolog (Tb), a model of the Escherichia coli homolog, and, most recently, a revised crystal structure of Tb-MscL (2OAR). To understand which of these structures represents a physiological conformation, we measured the impact of mutations to the C-terminal domain on the thermal stability of Tb-MscL using circular dichroism and performed molecular dynamics simulations of the original and the revised crystal structures of Tb-MscL. Our results imply that this region is helical and adopts an α-helical bundle conformation similar to that observed in the E. coli MscL model and the revised Tb-MscL crystal structure.
Item Type: | Article | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Related URLs: |
| |||||||||
ORCID: |
| |||||||||
Additional Information: | © 2008 The Biophysical Society. Received 6 December 2007, Accepted 23 January 2008, Available online 6 January 2009. This work was partially supported by the National Institutes of Health Program Project Grant GM-62532. Additional support was provided by the Office of the Dean at Wellesley College. Joshua A. Maurer and Donald E. Elmore contributed equally to this work. | |||||||||
Funders: |
| |||||||||
Issue or Number: | 12 | |||||||||
PubMed Central ID: | PMC2397327 | |||||||||
DOI: | 10.1529/biophysj.107.127365 | |||||||||
Record Number: | CaltechAUTHORS:20170409-064941478 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20170409-064941478 | |||||||||
Official Citation: | Maurer JA, Elmore DE, Clayton D, Xiong L, Lester HA, Dougherty DA. Confirming the Revised C-Terminal Domain of the MscL Crystal Structure. Biophysical Journal. 2008;94(12):4662-4667. doi:10.1529/biophysj.107.127365. | |||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 76420 | |||||||||
Collection: | CaltechAUTHORS | |||||||||
Deposited By: | 1Science Import | |||||||||
Deposited On: | 02 Apr 2018 21:39 | |||||||||
Last Modified: | 15 Nov 2021 16:59 |
Repository Staff Only: item control page