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Confirming the Revised C-Terminal Domain of the MscL Crystal Structure

Maurer, Joshua A. and Elmore, Donald E. and Clayton, Daniel and Xiong, Li and Lester, Henry A. and Dougherty, Dennis A. (2008) Confirming the Revised C-Terminal Domain of the MscL Crystal Structure. Biophysical Journal, 94 (12). pp. 4662-4667. ISSN 0006-3495. PMCID PMC2397327. doi:10.1529/biophysj.107.127365.

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The structure of the C-terminal domain of the mechanosensitive channel of large conductance (MscL) has generated significant controversy. As a result, several structures have been proposed for this region: the original crystal structure (1MSL) of the Mycobacterium tuberculosis homolog (Tb), a model of the Escherichia coli homolog, and, most recently, a revised crystal structure of Tb-MscL (2OAR). To understand which of these structures represents a physiological conformation, we measured the impact of mutations to the C-terminal domain on the thermal stability of Tb-MscL using circular dichroism and performed molecular dynamics simulations of the original and the revised crystal structures of Tb-MscL. Our results imply that this region is helical and adopts an α-helical bundle conformation similar to that observed in the E. coli MscL model and the revised Tb-MscL crystal structure.

Item Type:Article
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URLURL TypeDescription CentralArticle
Lester, Henry A.0000-0002-5470-5255
Dougherty, Dennis A.0000-0003-1464-2461
Additional Information:© 2008 The Biophysical Society. Received 6 December 2007, Accepted 23 January 2008, Available online 6 January 2009. This work was partially supported by the National Institutes of Health Program Project Grant GM-62532. Additional support was provided by the Office of the Dean at Wellesley College. Joshua A. Maurer and Donald E. Elmore contributed equally to this work.
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Wellesley CollegeUNSPECIFIED
Issue or Number:12
PubMed Central ID:PMC2397327
Record Number:CaltechAUTHORS:20170409-064941478
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Official Citation:Maurer JA, Elmore DE, Clayton D, Xiong L, Lester HA, Dougherty DA. Confirming the Revised C-Terminal Domain of the MscL Crystal Structure. Biophysical Journal. 2008;94(12):4662-4667. doi:10.1529/biophysj.107.127365.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:76420
Deposited By: 1Science Import
Deposited On:02 Apr 2018 21:39
Last Modified:15 Nov 2021 16:59

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