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Phosphatidyl inositol-linked forms of a murine class I MHC molecule expressed on Chinese hamster ovary cells retain peptide binding capability and alloreactivity

Fahnestock, Margaret L. and Dadgari, Joseph M. and McMillan, Minnie and Bjorkman, Pamela J. (1994) Phosphatidyl inositol-linked forms of a murine class I MHC molecule expressed on Chinese hamster ovary cells retain peptide binding capability and alloreactivity. International Immunology, 6 (2). pp. 307-314. ISSN 0953-8178. https://resolver.caltech.edu/CaltechAUTHORS:20170411-084740913

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Abstract

A gene encoding a phosphatidyl inosltoHlnked form of the murine class I MHC molecule H-2K^d was constructed and the protein expressed in Chinese hamster ovary cells together with murine or human β2-mlcroglobulln (β2m). The resulting llpld-llnked class I heterodlmers can be efficiently converted into a soluble form by treatment of transfected cells with a phosphollpase. Cells expressing K^d heterodlmers were characterized with respect to heavy chain levels at the cell surface, peptide binding, and recognition by K^d-speclflc antibodies and alloreactlve cytotoxlc T cells. All transfectants bound a ^3H-labeled K^d-restr1cted nonamer peptide, although more peptide bound to cells expressing the K^d/human βm combination, perhaps becafse of a greater number of empty molecules at the cell surface. A dissociation constant of 5 × 10^(−8) M derived by Scatchard analysis Is within the range expected for interactions of peptldes with class I MHC molecules. Alloreactlve cytotoxlc T cells which recognize wild-type K^d on murine cells lysed the hamster cells expressing llpld-llnked K^d without regard to the species of the βm light chain. These results indicated that the engineered llpld-llnked K^d molecule Is expressed at the cell surface, Is recognized by antibodies and T cells, and functions to bind peptide.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1093/intimm/6.2.307 DOIArticle
https://academic.oup.com/intimm/article-abstract/6/2/307/666306/Phosphatidyl-inositol-linked-forms-of-a-murine?redirectedFrom=fulltextPublisherArticle
ORCID:
AuthorORCID
Bjorkman, Pamela J.0000-0002-2277-3990
Additional Information:© 1994 Oxford University Press. Received: 11 April 1993; Accepted: 28 October 1993; Published: 01 February 1994.
Subject Keywords:allorecognition, H-2Kd, protein engineering
Issue or Number:2
Record Number:CaltechAUTHORS:20170411-084740913
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170411-084740913
Official Citation:Margaret L. Fahnestock, Joseph M. Dadgari, Minnie McMillan, Pamela J. Bjorkman; Phosphatidyl inositol-linked forms of a murine class I MHC molecule expressed on Chinese hamster ovary cells retain peptide binding capability and alloreactivity. Int Immunol 1994; 6 (2): 307-314. doi: 10.1093/intimm/6.2.307
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:76500
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:11 Apr 2017 16:55
Last Modified:03 Oct 2019 17:01

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