Lebrón, José A. and Shen, Huazhang and Bjorkman, Pamela J. and Ou, Susan (1999) Tolerization of adult mice to immunodominant proteins before monoclonal antibody production. Journal of Immunological Methods, 222 (1-2). pp. 59-63. ISSN 0022-1759. doi:10.1016/S0022-1759(98)00179-3. https://resolver.caltech.edu/CaltechAUTHORS:20170411-091833694
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Abstract
Generating monoclonal antibodies (mAbs) against one polypeptide chain of a heterodimeric protein can be difficult when the other chain is more immunogenic. To influence the immune response in favor of the less immunogenic protein, we rendered adult mice tolerant to the immunodominant protein using a procedure based on the phenomenon of high zone tolerance. We then immunized the tolerized mice with a heterodimeric protein containing the immunogenic protein and produced hybridomas in the usual way. Screening the hybridomas for reactivity against the immunodominant protein and against the heterodimer revealed that this tolerization procedure can result in an increase of hybridomas producing mAbs against the protein of interest by up to 90-fold. This method should be of general utility for the production of mAbs against weakly antigenic proteins in mixtures of antigens.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1999 Elsevier Science B.V. Received 31 July 1998, Accepted 11 September 1998, Available online 22 December 1998. | |||||||||
| Subject Keywords: | Tolerization; HFE; HLA-H; β2-microglobulin; CD94; NKG2A | |||||||||
| Issue or Number: | 1-2 | |||||||||
| DOI: | 10.1016/S0022-1759(98)00179-3 | |||||||||
| Record Number: | CaltechAUTHORS:20170411-091833694 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20170411-091833694 | |||||||||
| Official Citation: | José A Lebrón, Huazhang Shen, Pamela J Bjorkman, Susan Ou, Tolerization of adult mice to immunodominant proteins before monoclonal antibody production, Journal of Immunological Methods, Volume 222, Issues 1–2, 1 January 1999, Pages 59-63, ISSN 0022-1759, http://doi.org/10.1016/S0022-1759(98)00179-3. (http://www.sciencedirect.com/science/article/pii/S0022175998001793) | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 76502 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Tony Diaz | |||||||||
| Deposited On: | 11 Apr 2017 16:50 | |||||||||
| Last Modified: | 15 Nov 2021 17:00 |
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