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In Vitro Selection of mRNA Display Libraries Containing an Unnatural Amino Acid

Li, Shuwei and Millward, Steven and Roberts, Richard (2002) In Vitro Selection of mRNA Display Libraries Containing an Unnatural Amino Acid. Journal of the American Chemical Society, 124 (34). pp. 9972-9973. ISSN 0002-7863. doi:10.1021/ja026789q. https://resolver.caltech.edu/CaltechAUTHORS:20170414-133106247

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Abstract

The incorporation of unnatural amino acid into selectable, amplifiable peptide and protein libraries expands the chemical diversity of such libraries, thus considerably facilitating the process of obtaining ligands with improved properties (affinity, specificity, and function), particularly against therapeutically interesting targets. Here, we report that biocytin, a biotin derivative of lysine, can be inserted into an mRNA−protein fusion molecule through amber stop codon suppression. We also demonstrate that templates containing the codon corresponding to the biocytin tRNA (a UAG stop codon) can be enriched by iterative cycles of selection against a streptavidin agarose matrix.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja026789qDOIArticle
http://pubs.acs.org/doi/full/10.1021/ja026789qPublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja026789qPublisherSupporting Information
Additional Information:© 2002 American Chemical Society. Received 3 May 2002. Published online 31 July 2002. Published in print 1 August 2002. We thank Lintong Li for her help in preparing biocytin-charged-THG73. We thank Professor Dennis Dougherty's (Caltech) group for providing the dinucleotide dCpA and the plasmid for THG73 tRNA transcription. This work was supported by NIH Grant GM60416 (R.W.R).
Funders:
Funding AgencyGrant Number
NIHGM60416
Issue or Number:34
DOI:10.1021/ja026789q
Record Number:CaltechAUTHORS:20170414-133106247
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170414-133106247
Official Citation:In Vitro Selection of mRNA Display Libraries Containing an Unnatural Amino Acid Shuwei Li, Steven Millward, and Richard Roberts Journal of the American Chemical Society 2002 124 (34), 9972-9973 DOI: 10.1021/ja026789q
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:76572
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Apr 2017 20:50
Last Modified:15 Nov 2021 17:01

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