The Puromycin Route to Assess Stereo- and Regiochemical Constraints on Peptide Bond Formation in Eukaryotic Ribosomes

Starck, Shelley R. and Qi, Xin and Olsen, Brett N. and Roberts, Richard W. (2003) The Puromycin Route to Assess Stereo- and Regiochemical Constraints on Peptide Bond Formation in Eukaryotic Ribosomes. Journal of the American Chemical Society, 125 (27). pp. 8090-8091. ISSN 0002-7863. doi:10.1021/ja034817e. https://resolver.caltech.edu/CaltechAUTHORS:20170419-113623437

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Abstract

We synthesized a series of puromycin analogues to probe the chemical specificity of the ribosome in an intact eukaryotic translation system. These studies reveal that both d-enantiomers and β-amino acid analogues can be incorporated into protein, and provide a quantitative means to rank natural and unnatural residues. Modeling of a d-amino acid analogue into the 50S ribosomal subunit indicates that steric clash may provide part of the chiral discrimination. The data presented provide one metric of the chiral and regiospecificity of mammalian ribosomes.

Item Type:

Article

Related URLs:

URL	URL Type	Description
http://dx.doi.org/10.1021/ja034817e	DOI	Article
http://pubs.acs.org/doi/abs/10.1021/ja034817e	Publisher	Article
http://pubs.acs.org/doi/suppl/10.1021/ja034817e	Publisher	Supporting Information

ORCID:

Author	ORCID
Roberts, Richard W.	0000-0002-8587-5097

Additional Information:

© 2003 American Chemical Society. Received 22 February 2003. Published online 11 June 2003. Published in print 1 July 2003. This work was supported by NIH Grant (R01 60416) to R.W.R. and by NIH training Grant GM07616 (S.R.S.).

Funders:

Funding Agency	Grant Number
NIH	R01 60416
NIH	GM07616

Issue or Number:

DOI:

10.1021/ja034817e

Record Number:

CaltechAUTHORS:20170419-113623437

Persistent URL:

https://resolver.caltech.edu/CaltechAUTHORS:20170419-113623437

Official Citation:

The Puromycin Route to Assess Stereo- and Regiochemical Constraints on Peptide Bond Formation in Eukaryotic Ribosomes Shelley R. Starck, Xin Qi, Brett N. Olsen, and Richard W. Roberts Journal of the American Chemical Society 2003 125 (27), 8090-8091 DOI: 10.1021/ja034817e

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ID Code:

76686

Collection:

CaltechAUTHORS

Deposited By:

Ruth Sustaita

Deposited On:

19 Apr 2017 18:50

Last Modified:

15 Nov 2021 17:02

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