Miller, Jeremiah E. and Grǎdinaru, Cristian and Crane, Brian R. and Di Bilio, Angel J. and Wehbi, William A. and Un, Sun and Winkler, Jay R. and Gray, Harry B. (2003) Spectroscopy and Reactivity of a Photogenerated Tryptophan Radical in a Structurally Defined Protein Environment. Journal of the American Chemical Society, 125 (47). pp. 14220-14221. ISSN 0002-7863. doi:10.1021/ja037203i. https://resolver.caltech.edu/CaltechAUTHORS:20170419-130606164
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Abstract
Near-UV irradiation of structurally characterized [Re(I)(CO)_3(1,10-phenanthroline)(Q107H)](W48F/Y72F/H83Q/Y108W)AzM(II) [Az = Pseudomonas aeruginosa azurin, M = Cu, Zn]/[Co(NH_3)_5Cl]Cl_2 produces a tryptophan radical (W108•) with unprecedented kinetic stability. After rapid formation (k = 2.8 × 10^6 s^(-1)), the radical persists for more than 5 h at room temperature in the folded ReAzM(II) structure. The absorption spectrum of ReAz(W108•)M(II) exhibits maxima at 512 and 536 nm. Oxidation of K_4[Mo(CN)_8] by ReAz(W108•)Zn(II) places the W108•/W108 reduction potential in the protein above 0.8 V vs NHE.
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Additional Information: | © 2003 American Chemical Society. Received 10 July 2003. Published online 1 November 2003. Published in print 1 November 2003. Randy Villahermosa assisted with the transient absorption measurements. J.E.M. thanks the Parsons Foundation for a graduate fellowship. Work at Cornell was supported by NSF (MCB0133546); work at Caltech was supported by NIH (DK19038). | ||||||||||||
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Issue or Number: | 47 | ||||||||||||
DOI: | 10.1021/ja037203i | ||||||||||||
Record Number: | CaltechAUTHORS:20170419-130606164 | ||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20170419-130606164 | ||||||||||||
Official Citation: | Spectroscopy and Reactivity of a Photogenerated Tryptophan Radical in a Structurally Defined Protein Environment Jeremiah E. Miller, Cristian Grǎdinaru, Brian R. Crane, Angel J. Di Bilio, William A. Wehbi, Sun Un, Jay R. Winkler, and Harry B. Gray Journal of the American Chemical Society 2003 125 (47), 14220-14221 DOI: 10.1021/ja037203i | ||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
ID Code: | 76695 | ||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||
Deposited By: | Ruth Sustaita | ||||||||||||
Deposited On: | 19 Apr 2017 21:51 | ||||||||||||
Last Modified: | 15 Nov 2021 17:02 |
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