Rothman, Deborah M. and Petersson, E. James and Vázquez, M. Eugenio and Brandt, Gabriel S. and Dougherty, Dennis A. and Imperiali, Barbara (2005) Caged Phosphoproteins. Journal of the American Chemical Society, 127 (3). pp. 846-847. ISSN 0002-7863. doi:10.1021/ja043875c. https://resolver.caltech.edu/CaltechAUTHORS:20170420-063814654
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Abstract
We present the chemical and biological synthesis of caged phosphoproteins using the in vitro nonsense codon suppression methodology. Specifically, phosphoamino acid analogues of serine, threonine, and tyrosine with a single photocleavable o-nitrophenylethyl caging group were synthesized as the amino acyl tRNA adducts for insertion into full-length proteins. For this purpose, a novel phosphitylating agent was developed. The successful incorporation of these bulky and charged amino acids into the α-subunit of the nicotinic acetyl choline receptor (nAChR) and the vasodilator-stimulated phosphoprotein (VASP) using an in vitro translation system is reported.
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| Additional Information: | © 2005 American Chemical Society. Received 7 October 2004. Published online 30 December 2004. Published in print 1 January 2005. The work has been supported by the Cell Migration Consortium Grant GM64346 to B.I., NS-34407 to D.A.D, and the HFSP Foundation for M.E.V. The authors thank Professor F. Gertler for the VASP gene and antibody. | ||||||||||||
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| Issue or Number: | 3 | ||||||||||||
| DOI: | 10.1021/ja043875c | ||||||||||||
| Record Number: | CaltechAUTHORS:20170420-063814654 | ||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20170420-063814654 | ||||||||||||
| Official Citation: | Caged Phosphoproteins Deborah M. Rothman, E. James Petersson, M. Eugenio Vázquez, Gabriel S. Brandt, Dennis A. Dougherty, and Barbara Imperiali Journal of the American Chemical Society 2005 127 (3), 846-847 DOI: 10.1021/ja043875c | ||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
| ID Code: | 76742 | ||||||||||||
| Collection: | CaltechAUTHORS | ||||||||||||
| Deposited By: | Ruth Sustaita | ||||||||||||
| Deposited On: | 20 Apr 2017 16:29 | ||||||||||||
| Last Modified: | 15 Nov 2021 17:02 |
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