CaltechAUTHORS
  A Caltech Library Service

Caged Phosphoproteins

Rothman, Deborah M. and Petersson, E. James and Vázquez, M. Eugenio and Brandt, Gabriel S. and Dougherty, Dennis A. and Imperiali, Barbara (2005) Caged Phosphoproteins. Journal of the American Chemical Society, 127 (3). pp. 846-847. ISSN 0002-7863. doi:10.1021/ja043875c. https://resolver.caltech.edu/CaltechAUTHORS:20170420-063814654

[img] PDF - Supplemental Material
See Usage Policy.

29MB

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20170420-063814654

Abstract

We present the chemical and biological synthesis of caged phosphoproteins using the in vitro nonsense codon suppression methodology. Specifically, phosphoamino acid analogues of serine, threonine, and tyrosine with a single photocleavable o-nitrophenylethyl caging group were synthesized as the amino acyl tRNA adducts for insertion into full-length proteins. For this purpose, a novel phosphitylating agent was developed. The successful incorporation of these bulky and charged amino acids into the α-subunit of the nicotinic acetyl choline receptor (nAChR) and the vasodilator-stimulated phosphoprotein (VASP) using an in vitro translation system is reported.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja043875cDOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja043875cPublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja043875cPublisherSupporting Information
ORCID:
AuthorORCID
Petersson, E. James0000-0003-3854-9210
Dougherty, Dennis A.0000-0003-1464-2461
Additional Information:© 2005 American Chemical Society. Received 7 October 2004. Published online 30 December 2004. Published in print 1 January 2005. The work has been supported by the Cell Migration Consortium Grant GM64346 to B.I., NS-34407 to D.A.D, and the HFSP Foundation for M.E.V. The authors thank Professor F. Gertler for the VASP gene and antibody.
Funders:
Funding AgencyGrant Number
NIHGM64346
NIHNS-34407
Human Frontier Science ProgramUNSPECIFIED
Issue or Number:3
DOI:10.1021/ja043875c
Record Number:CaltechAUTHORS:20170420-063814654
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170420-063814654
Official Citation:Caged Phosphoproteins Deborah M. Rothman, E. James Petersson, M. Eugenio Vázquez, Gabriel S. Brandt, Dennis A. Dougherty, and Barbara Imperiali Journal of the American Chemical Society 2005 127 (3), 846-847 DOI: 10.1021/ja043875c
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:76742
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:20 Apr 2017 16:29
Last Modified:15 Nov 2021 17:02

Repository Staff Only: item control page