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Attenuation of Cellular Inflammation Using Glucocorticoid-Functionalized Copolymers

Chow, Edward K. and Pierstorff, Erik and Cheng, Genhong and Tai, Yu-Chong and Ho, Dean (2007) Attenuation of Cellular Inflammation Using Glucocorticoid-Functionalized Copolymers. In: 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems. IEEE , Piscataway, NJ, pp. 1039-1043. ISBN 1-4244-0609-9.

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This work has demonstrated the functionalization of an amphiphilic diblock copolymer, comprised of polyethylene oxide-polymethyl methacrylate (PEO-PMMA), as well as a triblock copolymer comprised of polymethyloxazoline-polydimethylsiloxane-polymethyloxazoline(PMOXA-PDMS-PM OXA) with the dexamethasone (Dex) glucocorticoid anti-inflammatory. Interfacial deposition of the copolymer and the Dex molecules and subsequent transfer of the hybrid materials to solid substrates were characterized to evaluate the potential of utilizing this composite material as a suppressor of cyto-inflammation to enhance implant biocompatibility. Given the extremely thin dimensions of the film (~4nm), this material would have negligible impact upon the size of the coated device to preclude biological stress. The composite films were interfaced with the RAW264.7 murine macrophages which served as a model cell line for the evaluation of nuclear factor-kappaB (NF-KB)-induced production of a host of inflammatory cytokines including interleukin-6, interleukin-12, tumor necrosis factor-alpha (TNFalpha), as well as the inducible nitric oxide synthase signaling factor which is known to be involved with stress-related processes such as neuronal damage. Lipopolysaccharide or LPS is a component of bacterial membranes that elicits cellular stress following application to RAW cell cultures. Following the induced stress response, significant reductions in the expression of genes associated with the aforementioned cytokines and signaling molecules indicated that macrophages in direct contact with the functionalized copolymer were able to collect Dex that was released from within the polymer network to attenuate cyto-inflammation mechanisms. This composite membrane represents a medically-relevant technology to promote chronic implant functionality and preclusion of bio-fouling.

Item Type:Book Section
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Tai, Yu-Chong0000-0001-8529-106X
Additional Information:© 2007 IEEE. D. Ho would like to acknowledge generous support from the National Institute of Allergy and Infectious Diseases of the National Institutes of Health. E. Chow was supported by the Ruth L. Kirschstein National Research Award GM07185.
Funding AgencyGrant Number
NIH Predoctoral FellowshipGM07185
Subject Keywords:cell regulation, copolymer, biomaterials
Record Number:CaltechAUTHORS:20170420-120436143
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Official Citation:E. K. Chow, E. Pierstorff, G. Cheng, Y. C. Tai and D. Ho, "Attenuation of Cellular Inflammation Using Glucocorticoid-Functionalized Copolymers," 2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, Bangkok, 2007, pp. 1039-1043. doi: 10.1109/NEMS.2007.352195
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:76772
Deposited By: Kristin Buxton
Deposited On:20 Apr 2017 20:49
Last Modified:15 Nov 2021 17:02

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