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Redox Couples of Inducible Nitric Oxide Synthase

Udit, Andrew K. and Belliston-Bittner, Wendy and Glazer, Edith C. and Nguyen, Yen Hoang Le and Gillan, James M. and Hill, Michael G. and Marletta, Michael A. and Goodin, David B. and Gray, Harry B. (2005) Redox Couples of Inducible Nitric Oxide Synthase. Journal of the American Chemical Society, 127 (32). pp. 11212-11213. ISSN 0002-7863. PMCID PMC2552398. https://resolver.caltech.edu/CaltechAUTHORS:20170421-153121728

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Abstract

We report direct electrochemistry of the iNOS heme domain in a DDAB film on the surface of a basal plane graphite electrode. Cyclic voltammetry reveals Fe^(III/II) and Fe^(II/I) couples at −191 and −1049 mV (vs Ag/AgCl). Imidazole and carbon monoxide in solution shift the Fe^(III/II) potential by +20 and +62 mV, while the addition of dioxygen results in large catalytic waves at the onset of Fe^(III) reduction. Voltammetry at higher scan rates (with pH variations) reveals that the Fe^(III/II) cathodic peak can be resolved into two components, which are attributable to Fe^(III/II) couples of five- and six-coordinate hemes. Digital simulation of our experimental data implicates water dissociation from the heme as a gating mechanism for ET in iNOS.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja0520114DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja0520114PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja0520114PublisherSupporting Information
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2552398PubMed CentralArticle
Additional Information:© 2005 American Chemical Society. Received March 29, 2005. Publication Date (Web): July 20, 2005. Supported by NSERC (Canada) (A.K.U.), HHMI (A.K.U.), NIH (DK19038; GM070868), the Packard Foundation (M.G.H.), the Ellison Medical Foundation (Senior Scholar Award in Aging to H.B.G.), and the Parsons Foundation (W.B.B.); S. Luzzi (Berkeley) assisted with protein expression and purification.
Funders:
Funding AgencyGrant Number
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
NIHDK19038
NIHGM070868
David and Lucile Packard FoundationUNSPECIFIED
Ellison Medical FoundationUNSPECIFIED
Ralph M. Parsons FoundationUNSPECIFIED
Issue or Number:32
PubMed Central ID:PMC2552398
Record Number:CaltechAUTHORS:20170421-153121728
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170421-153121728
Official Citation:Redox Couples of Inducible Nitric Oxide Synthase Andrew K. Udit, Wendy Belliston-Bittner, Edith C. Glazer, Yen Hoang Le Nguyen, James M. Gillan, Michael G. Hill, Michael A. Marletta, David B. Goodin, and Harry B. Gray Journal of the American Chemical Society 2005 127 (32), 11212-11213 DOI: 10.1021/ja0520114
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:76830
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:24 Apr 2017 14:36
Last Modified:03 Oct 2019 17:50

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