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The effect of HeLa cell cytoplasm on the rate of sedimentation of RNA

Girard, Marc and Baltimore, David (1966) The effect of HeLa cell cytoplasm on the rate of sedimentation of RNA. Proceedings of the National Academy of Sciences of the United States of America, 56 (3). pp. 999-1002. ISSN 0027-8424. doi:10.1073/pnas.56.3.999.

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Ordinarily, sedimentation analysis of RNA preparations is carried out in simple aqueous solutions. Recently, numerous investigators have carried out experiments where RNA's in cytoplasmic extracts from various types of cells have been analyzed by sedimentation through gradients of sucrose. The implicit assumption has been made that the presence of the cytoplasmic extract does not in itself affect the rate of sedimentation of the RNA. However, we have recently found that some component of HeLa cell cytoplasmic extracts causes a 1.5-2-fold increase in the sedimentation rate of a number of species of RNA. Since this result bears on the interpretation of many types of experiments, we will report the phenomenon at this time, even though we know little about its mechanism.

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Baltimore, David0000-0001-8723-8190
Additional Information:© 1966 by the National Academy of Sciences. Communicated by Renato Dulbecco, July 13, 1966. It is a pleasure to acknowledge the technical assistance of Mr. Stanley Read. Supported by grant CA 07592 from the National Cancer Institute, USPHS.
Issue or Number:3
Record Number:CaltechAUTHORS:GIRpnas66
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7684
Deposited By: Tony Diaz
Deposited On:22 Mar 2007
Last Modified:08 Nov 2021 20:44

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