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Localized repressors delineate the neurogenic ectoderm in the early Drosophila embryo

Stathopoulos, Angelike and Levine, Michael (2005) Localized repressors delineate the neurogenic ectoderm in the early Drosophila embryo. Developmental Biology, 280 (2). pp. 482-493. ISSN 0012-1606. doi:10.1016/j.ydbio.2005.02.003. https://resolver.caltech.edu/CaltechAUTHORS:20170424-124155343

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Abstract

The Dorsal gradient produces sequential patterns of gene expression across the dorsoventral axis of early embryos, thereby establishing the presumptive mesoderm, neuroectoderm, and dorsal ectoderm. Spatially localized repressors such as Snail and Vnd exclude the expression of neurogenic genes in the mesoderm and ventral neuroectoderm, respectively. However, no repressors have been identified that establish the dorsal limits of neurogenic gene expression. To investigate this issue, we have conducted an analysis of the ind gene, which is selectively expressed in lateral regions of the presumptive nerve cord. A novel silencer element was identified within the ind enhancer that is essential for eliminating expression in the dorsal ectoderm. Evidence is presented that the associated repressor can function over long distances to silence neighboring enhancers. The ind enhancer also contains a variety of known activator and repressor elements. We propose a model whereby Dorsal and EGF signaling, together with the localized Schnurri repressor, define a broad domain of ind expression throughout the entire presumptive neuroectoderm. The ventral limits of gene expression are defined by the Snail and Vnd repressors, while the dorsal border is established by the newly defined silencer element.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://doi.org/10.1016/j.ydbio.2005.02.003DOIArticle
http://www.sciencedirect.com/science/article/pii/S001216060500076XPublisherArticle
ORCID:
AuthorORCID
Stathopoulos, Angelike0000-0001-6597-2036
Additional Information:© 2005 Elsevier Inc. Received 3 February 2005, Revised 3 February 2005, Accepted 3 February 2005, Available online 5 March 2005. We are grateful to Bergin Tam, Jason Zemansky, and especially Robert Zinzen for help with in situ hybridizations and confocal imaging. We also thank John Cowden for providing the ind hybridization probe, and Eric Davidson and Albert Erives for helpful discussions. This work was supported by grants from the NIH (GM34431 and GM46638).
Funders:
Funding AgencyGrant Number
NIHGM34431
NIHGM46638
Subject Keywords:Dorsoventral patterning; Drosophila; Repressor; Neurogenic ectoderm; Dorsal; Ind; Vnd; Schnurri
Issue or Number:2
DOI:10.1016/j.ydbio.2005.02.003
Record Number:CaltechAUTHORS:20170424-124155343
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170424-124155343
Official Citation:Angelike Stathopoulos, Michael Levine, Localized repressors delineate the neurogenic ectoderm in the early Drosophila embryo, Developmental Biology, Volume 280, Issue 2, 15 April 2005, Pages 482-493, ISSN 0012-1606, http://doi.org/10.1016/j.ydbio.2005.02.003. (http://www.sciencedirect.com/science/article/pii/S001216060500076X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:76856
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:24 Apr 2017 20:02
Last Modified:15 Nov 2021 17:03

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