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Calcineurin acts through the CRZ1/TCN1-encoded transcription factor to regulate gene expression in yeast

Stathopoulos, Angelike M. and Cyert, Martha S. (1997) Calcineurin acts through the CRZ1/TCN1-encoded transcription factor to regulate gene expression in yeast. Genes and Development, 11 (24). pp. 3432-3444. ISSN 0890-9369. PMCID PMC316814. https://resolver.caltech.edu/CaltechAUTHORS:20170424-130811787

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Abstract

Calcineurin is a conserved Ca^(2+)/calmodulin-dependent protein phosphatase that plays a critical role in Ca^(2+) signaling. We describe new components of a calcineurin-mediated response in yeast, the Ca^(2+)-induced transcriptional activation ofFKS2, which encodes a β-1,3 glucan synthase. A 24-bp region of the FKS2 promoter was defined as sufficient to confer calcineurin-dependent transcriptional induction on a minimal promoter in response to Ca^(2+) and was named CDRE (forcalcineurin-dependentresponse element). The product ofCRZ1 (YNL027w) was identified as an activator of CDRE-driven transcription. Crz1p contains zinc finger motifs and binds specifically to the CDRE. Genetic analysis revealed that crz1Δ mutant cells exhibit several phenotypes similar to those of calcineurin mutants and that overexpression of CRZ1 in calcineurin mutants suppressed these phenotypes. These results suggest that Crz1p functions downstream of calcineurin to effect multiple calcineurin-dependent responses. Moreover, the calcineurin-dependent transcriptional induction of FKS2 in response to Ca^(2+), α-factor, and Na^+ was found to require CRZ1. In addition, we found that the calcineurin-dependent transcriptional regulation ofPMR2 and PMC1 required CRZ1. However, transcription of PMR2 and PMC1 was activated by only a subset of the treatments that activated FKS2 transcription. Thus, in response to multiple signals, calcineurin acts through the Crz1p transcription factor to differentially regulate the expression of several target genes in yeast.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1101/gad.11.24.3432DOIArticle
http://genesdev.cshlp.org/content/11/24/3432PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC316814PubMed CentralArticle
ORCID:
AuthorORCID
Stathopoulos, Angelike M.0000-0001-6597-2036
Additional Information:© 1997 Cold Spring Harbor Laboratory Press. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, http://creativecommons.org/licenses/by-nc/4.0/). Received September 15, 1997; revised version accepted October 14, 1997. We give special thanks to Tai Roe for providing pAMS312 and Daphna Axelrod for providing pAMS317. We also thank Phil Garrett-Engele, Joachim Li, Francisco Rubio, Philip James, Johannes Hegemann, and Scott Erdman for providing other invaluable reagents. In addition, we wish to thank Mohamad Moghadam and Bob Fisher for help with the gel-shift experiments and Kyle Cunningham for sharing results before publication. We are grateful to members of the Cyert laboratory for helpful discussions and support and to Tai Roe, Kirstie Saltsman, James Withee, Tim Stearns, Pamela Carroll, and Tamara Pozos for critical reading of the manuscript. M.S.C. is supported by a biomedical scholar award from the Lucille P. Markey Charitable Trust, National Science Foundation Young Investigator award MCB-9357017, funds from the Procter and Gamble Company, and National Institutes of Health (NIH) research grant GM48729. A.M.S. is supported by NIH training grant 5T32GM07276-22. The publication costs of this article were defrayed in part by payment of page charges. This article must therefore be hereby marked ‘‘advertisement’’ in accordance with 18 USC section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
Lucille P. Markey Charitable TrustUNSPECIFIED
NSFMCB-9357017
Procter and Gamble CompanyUNSPECIFIED
NIHGM48729
NIH Predoctoral Fellowship5T32GM07276-22
Subject Keywords:S. cerevisiae; calcineurin; calcium signaling; transcriptional activation; cell wall maintenance; ion homeostasis
Issue or Number:24
PubMed Central ID:PMC316814
Record Number:CaltechAUTHORS:20170424-130811787
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170424-130811787
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:76862
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:24 Apr 2017 20:34
Last Modified:03 Oct 2019 17:50

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