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Yeast calcineurin regulates nuclear localization of the Crz1p transcription factor through dephosphorylation

Stathopoulos-Gerontides, Angelike and Guo, Jim Jun and Cyert, Martha S. (1999) Yeast calcineurin regulates nuclear localization of the Crz1p transcription factor through dephosphorylation. Genes and Development, 13 (7). pp. 798-803. ISSN 0890-9369. PMCID PMC316598.

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Calcineurin, a Ca^(2+)/calmodulin dependent protein phosphatase, regulates Ca^(2+)-dependent processes in a wide variety of cells. In the yeast, Saccharomyces cerevisiae, calcineurin effects Ca^(2+)-dependent changes in gene expression through regulation of the Crz1p transcription factor. We show here that calcineurin dephosphorylates Crz1p and that this results in translocation of Crz1p to the nucleus. We identify a region of Crz1p that is required for calcineurin-dependent regulation of its phosphorylation, localization, and activity, and show that this region has significant sequence simlarity to a portion of NF-AT, a family of mammalian transcription factors whose localization is also regulated by calcineurin. Thus, the mechanism of Ca^(2+)/calcineurin-dependent signaling shows remarkable conservation between yeast and mammalian cells.

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URLURL TypeDescription CentralArticle
Stathopoulos-Gerontides, Angelike0000-0001-6597-2036
Additional Information:© 1999 Cold Spring Harbor Laboratory Press. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, Received January 25, 1999; accepted February 5, 1999. We thank Jerry Crabtree, Jeremy Thorner, and members of the Cyert laboratory for comments on the manuscript, Fujisawa for supplying FK506, Merck for supplying FK520, and Pam Silver for supplying anti-GFP antibody. We thank Kim Williams for identifying the sequence similarity between Crz1p and NF-AT. We thank Renée Polizotto and John Gerontides for helpful discussion and Tim Stearns for his generous advice concerning fluorescence microscopy. This work was supported from the following sources: National Institutes of Health research grant GM-48728, The National Science Foundation Young Investigator Award MCB-9357017, biomedical scholar award 92-42 from the Lucille P. Markey Charitable Trust, and funds from Proctor and Gamble (all to M.S.C.). The publication costs of this article were defrayed in part by payment of page charges. This article must therefore be hereby marked ‘advertisement’ in accordance with 18 USC section 1734 solely to indicate this fact.
Funding AgencyGrant Number
Lucille P. Markey Charitable Trust92-42
Proctor and GambleUNSPECIFIED
Issue or Number:7
PubMed Central ID:PMC316598
Record Number:CaltechAUTHORS:20170424-131537181
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:76863
Deposited By: Tony Diaz
Deposited On:24 Apr 2017 20:32
Last Modified:03 Oct 2019 17:50

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