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Fluorescent Labeling, Sensing and Differentiation of Leukocytes from Undiluted Whole Blood Samples

Zheng, Siyang and Lin, Jeffrey Chun-Hui and Kasdan, Harvey L. and Tai, Yu-Chong (2007) Fluorescent Labeling, Sensing and Differentiation of Leukocytes from Undiluted Whole Blood Samples. In: TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference. IEEE , Piscataway, NJ, pp. 807-810. ISBN 1-4244-0841-5 . http://resolver.caltech.edu/CaltechAUTHORS:20170425-154716463

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Abstract

In this paper, we demonstrated leukocyte labeling, sensing and differentiation from undiluted human whole blood samples with microfabricated devices. A challenging issue in leukocyte sensing from blood samples is the required high dilution level, which is used mainly to prevent interference from the overwhelmingly outnumbered erythrocytes. Dilution is undesirable for micro hemacytometers. It not only increases sample volume and processing time, but also requires mixing and buffer storage for on- chip implementation. Unlike commercial bulk instruments and previous efforts by other groups, we completely eliminated the requirement for dilution by staining leukocytes specifically with fluorescent dye acridine orange (AO) in undiluted human whole blood and then sensing them in microfluidic devices. Green fluorescent signal centered at 525 nm was used for leukocyte count and red fluorescent signal centered at 650 nm was used for leukocyte differentiation. Throughput of one hundred leukocytes per second was achieved, which means operation time for one sample only requires several seconds.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1109/SENSOR.2007.4300253DOIArticle
http://ieeexplore.ieee.org/document/4300253/PublisherArticle
ORCID:
AuthorORCID
Tai, Yu-Chong0000-0001-8529-106X
Additional Information:© 2007 IEEE. The authors would like to thank for all the members at Caltech Micromachining Group for their value assistance. Also the authors would like to thank for Mr. Zhaoyu Zhang from California Institute of Technology for his discussion and assistance. This work was supported by the National Space Biomedical Research Institute through NASA NCC 9-58.
Funders:
Funding AgencyGrant Number
NASANCC 9-58
Subject Keywords:Leukocyte, Fluorescent, Sensing, Differentiation, Acridine Orange
Record Number:CaltechAUTHORS:20170425-154716463
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20170425-154716463
Official Citation:S. Zheng, J. C. H. Lin, H. L. Kasdan and Y. C. Tai, "Fluorescent Labeling, Sensing and Differentiation of Leukocytes from Undiluted Whole Blood Samples," TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference, Lyon, 2007, pp. 807-810. doi: 10.1109/SENSOR.2007.4300253
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:76922
Collection:CaltechAUTHORS
Deposited By: Kristin Buxton
Deposited On:26 Apr 2017 01:44
Last Modified:20 Mar 2019 18:43

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