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Protein−Surfactant Film Voltammetry of Wild-Type and Mutant Cytochrome P450 BM3

Udit, Andrew K. and Hindoyan, Nareen and Hill, Michael G. and Arnold, Frances H. and Gray, Harry B. (2005) Protein−Surfactant Film Voltammetry of Wild-Type and Mutant Cytochrome P450 BM3. Inorganic Chemistry, 44 (12). pp. 4109-4111. ISSN 0020-1669. doi:10.1021/ic0483747. https://resolver.caltech.edu/CaltechAUTHORS:20170426-070838205

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Abstract

We are investigating the redox chemistry of wild-type (WT) and mutant (1-12G) cytochrome P450 BM3. Absorption spectra in solution feature the FeIII Soret at 418 nm for WT and a split Soret for 1-12G at 390 and 418 nm. Voltammetry of the proteins within DDAPSS films on the surface of carbon electrodes reveal nearly identical Fe^(III/II) potentials (approximately −200 mV vs Ag/AgCl), but significant differences in k°, 250 vs 30 s^(-1), and Fe^(III/II)−CO potentials, −140 vs −115 mV, for WT vs 1-12G. Catalytic reduction of dioxygen by the proteins on rotating-disk electrodes was analyzed using Levich and Koutecky−Levich treatments. The data reveal 1-12G n and k_(obs) values that are, respectively, 1.7 and 0.07 times those of WT, suggesting that the two proteins differ strikingly in their reactions with dioxygen.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ic0483747DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ic0483747PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ic0483747PublisherSupporting Information
ORCID:
AuthorORCID
Arnold, Frances H.0000-0002-4027-364X
Gray, Harry B.0000-0002-7937-7876
Additional Information:© 2005 American Chemical Society. Received 17 November 2004. Published online 11 May 2005. Published in print 1 June 2005. E. Blair and P. J. Farmer (UC Irvine) for assistance with film preparation; M. W. Peters and P. Meinhold (Caltech) for helpful discussions; and NSERC (Canada) (A.K.U.), NIH (H.B.G.), and the David and Lucille Packard Foundation (M.G.H.) for research support.
Funders:
Funding AgencyGrant Number
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
NIHUNSPECIFIED
David and Lucille Packard FoundationUNSPECIFIED
Issue or Number:12
DOI:10.1021/ic0483747
Record Number:CaltechAUTHORS:20170426-070838205
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170426-070838205
Official Citation:Protein−Surfactant Film Voltammetry of Wild-Type and Mutant Cytochrome P450 BM3 Andrew K. Udit, Nareen Hindoyan, Michael G. Hill, Frances H. Arnold, and Harry B. Gray Inorganic Chemistry 2005 44 (12), 4109-4111 DOI: 10.1021/ic0483747
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:76933
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:26 Apr 2017 16:01
Last Modified:15 Nov 2021 17:03

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