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Protein−Surfactant Film Voltammetry of Wild-Type and Mutant Cytochrome P450 BM3

Udit, Andrew K. and Hindoyan, Nareen and Hill, Michael G. and Arnold, Frances H. and Gray, Harry B. (2005) Protein−Surfactant Film Voltammetry of Wild-Type and Mutant Cytochrome P450 BM3. Inorganic Chemistry, 44 (12). pp. 4109-4111. ISSN 0020-1669. doi:10.1021/ic0483747.

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We are investigating the redox chemistry of wild-type (WT) and mutant (1-12G) cytochrome P450 BM3. Absorption spectra in solution feature the FeIII Soret at 418 nm for WT and a split Soret for 1-12G at 390 and 418 nm. Voltammetry of the proteins within DDAPSS films on the surface of carbon electrodes reveal nearly identical Fe^(III/II) potentials (approximately −200 mV vs Ag/AgCl), but significant differences in k°, 250 vs 30 s^(-1), and Fe^(III/II)−CO potentials, −140 vs −115 mV, for WT vs 1-12G. Catalytic reduction of dioxygen by the proteins on rotating-disk electrodes was analyzed using Levich and Koutecky−Levich treatments. The data reveal 1-12G n and k_(obs) values that are, respectively, 1.7 and 0.07 times those of WT, suggesting that the two proteins differ strikingly in their reactions with dioxygen.

Item Type:Article
Related URLs:
URLURL TypeDescription Information
Arnold, Frances H.0000-0002-4027-364X
Gray, Harry B.0000-0002-7937-7876
Additional Information:© 2005 American Chemical Society. Received 17 November 2004. Published online 11 May 2005. Published in print 1 June 2005. E. Blair and P. J. Farmer (UC Irvine) for assistance with film preparation; M. W. Peters and P. Meinhold (Caltech) for helpful discussions; and NSERC (Canada) (A.K.U.), NIH (H.B.G.), and the David and Lucille Packard Foundation (M.G.H.) for research support.
Funding AgencyGrant Number
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
David and Lucille Packard FoundationUNSPECIFIED
Issue or Number:12
Record Number:CaltechAUTHORS:20170426-070838205
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Official Citation:Protein−Surfactant Film Voltammetry of Wild-Type and Mutant Cytochrome P450 BM3 Andrew K. Udit, Nareen Hindoyan, Michael G. Hill, Frances H. Arnold, and Harry B. Gray Inorganic Chemistry 2005 44 (12), 4109-4111 DOI: 10.1021/ic0483747
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:76933
Deposited By: Ruth Sustaita
Deposited On:26 Apr 2017 16:01
Last Modified:15 Nov 2021 17:03

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