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Dip-Pen Nanolithography of Reactive Alkoxysilanes on Glass

Jung, Hyungil and Kulkarni, Rajan and Collier, C. Patrick (2003) Dip-Pen Nanolithography of Reactive Alkoxysilanes on Glass. Journal of the American Chemical Society, 125 (40). pp. 12096-12097. ISSN 0002-7863. doi:10.1021/ja0363720.

[img] PDF (Tapping mode AFM image of MPTMS monolayer, methods used for biotin/streptavidin conjugation, LFM, and corresponding fluorescent images) - Supplemental Material
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The use of organofunctional silane chemistry is a flexible and general method for immobilizing biomolecules on silicon oxide surfaces, including fabricating DNA, small-molecule, and protein microarrays. The biggest hurdle in employing dip-pen nanolithography (DPN) for extending this general approach to the nanoscopic domain is the tendency of trialkoxy- and trichlorosilanes to rapidly polymerize due to hydrolysis reactions. The control of the local water concentration between the substrate surface and the scanning AFM tip is critical, both to the physical and chemical processes involved in DPN writing and to the ability to form well-defined thin layers of reactive silanes without extensive polymerization induced disorder. We found that we could control the degree of polymerization through careful choice of the alkoxysilane used as the “ink” for DPN and through control of the relative humidity during inking and writing with the coated AFM tip. As a proof-of-principle, we demonstrate that areas patterned with an alkoxysilane on glass with DPN are functional for subsequent immobilization of fluorescently labeled streptavidin via covalent attachment of biotin. This preliminary result sets the stage for the ability to capture proteins in their fully hydrated state from buffered solution, by molecular recognition onto previously written reactive nanoscopic regions on oxidized silicon and glass.

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Additional Information:© 2003 American Chemical Society. Received May 27, 2003. Publication Date (Web): September 11, 2003. This work was supported by Caltech startup funds. We thank Dr. Maria Esplandiu for help with tapping mode AFM imaging in water.
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Issue or Number:40
Record Number:CaltechAUTHORS:20170426-075707609
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Official Citation:Dip-Pen Nanolithography of Reactive Alkoxysilanes on Glass Hyungil Jung, Rajan Kulkarni, and C. Patrick Collier Journal of the American Chemical Society 2003 125 (40), 12096-12097 DOI: 10.1021/ja0363720
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:76936
Deposited By: Tony Diaz
Deposited On:26 Apr 2017 15:56
Last Modified:15 Nov 2021 17:04

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