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Reduction of Dioxygen Catalyzed by Pyrene-Wired Heme Domain Cytochrome P450 BM3 Electrodes

Udit, Andrew K. and Hill, Michael G. and Bittner, V. Garrett and Arnold, Frances H. and Gray, Harry B. (2004) Reduction of Dioxygen Catalyzed by Pyrene-Wired Heme Domain Cytochrome P450 BM3 Electrodes. Journal of the American Chemical Society, 126 (33). pp. 10218-10219. ISSN 0002-7863. doi:10.1021/ja0466560. https://resolver.caltech.edu/CaltechAUTHORS:20170426-144912333

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Abstract

We have electronically wired the cytochrome P450 BM3 heme domain to a graphite electrode with the use of a pyrene-terminated tether. AFM images clearly reveal that pyrene-wired enzyme molecules are adsorbed to the electrode surface. The enzyme-electrode system undergoes rapid and reversible electron transfer, displaying a standard rate constant higher than that of any other P450-electrode system. We also show that the graphite-pyrene-BM3 system catalyzes the four-electron reduction of dioxygen to water.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja0466560DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja0466560PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja0466560PublisherSupporting Information
ORCID:
AuthorORCID
Arnold, Frances H.0000-0002-4027-364X
Gray, Harry B.0000-0002-7937-7876
Additional Information:© 2004 American Chemical Society. Received June 7, 2004. Publication Date (Web): August 3, 2004. We thank J. S. Magyar and J. R. Winkler (Caltech), T. L. Poulos (U.C. Irvine), and E. M. Spain (Occidental College) for helpful discussions; C. P. Collier (Caltech) for assistance with the AFM; NSF (H.B.G.), NSERC (Canada) (A.K.U.), and David and Lucile Packard Foundation (M.G.H.) for research support.
Funders:
Funding AgencyGrant Number
NSFUNSPECIFIED
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
David and Lucile Packard FoundationUNSPECIFIED
Issue or Number:33
DOI:10.1021/ja0466560
Record Number:CaltechAUTHORS:20170426-144912333
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170426-144912333
Official Citation:Reduction of Dioxygen Catalyzed by Pyrene-Wired Heme Domain Cytochrome P450 BM3 Electrodes Andrew K. Udit, Michael G. Hill, V. Garrett Bittner, Frances H. Arnold, and Harry B. Gray Journal of the American Chemical Society 2004 126 (33), 10218-10219 DOI: 10.1021/ja0466560
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:76960
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:26 Apr 2017 23:05
Last Modified:15 Nov 2021 17:04

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