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Stability of lipid vesicles in tissues of the mouse: A gamma-ray perturbed angular correlation study

Mauk, Marcia R. and Gamble, Ronald C. (1979) Stability of lipid vesicles in tissues of the mouse: A gamma-ray perturbed angular correlation study. Proceedings of the National Academy of Sciences of the United States of America, 76 (2). pp. 765-769. ISSN 0027-8424. doi:10.1073/pnas.76.2.765.

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The rate of phospholipid vesicle disruption in specific tissues of the mouse was followed by gamma-ray perturbed angular correlation (PAC) spectroscopy. In these studies, high levels of 111In-nitrilotriacetic acid complex are contained in unilamellar vesicles consisting of distearoyl phosphatidylcholine, cholesterol, and small amounts of other lipids which modify the surface properties. The PAC technique monitors the extent of vesicle breakup by measuring a time-integrated perturbation factor, <G22(∞)>. As the vesicles are broken open in vivo, the released 111In3+ ions quickly bind to macromolecules and the <G22(∞)> value decreases substantially. After administration of vesicles by various routes (intravenous, intraperitoneal, subcutaneous, and oral), the radioactivity and <G22(∞)> values were determined for several tissues at intervals up to 24 hr. We conclude from these data that (i) the PAC technique in conjunction with standard gamma counting methods provides unique information on the condition and location of vesicles in specific tissues, (ii) significant differences in vesicle integrity are found in various tissues, and (iii) both the means of administration and the presence of surface charge affect the vesicle stability and distribution. The carbohydrate analogues of cholesterol affect vesicle stability but not distribution.

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Additional Information:© 1979 by the National Academy of Sciences. Communicated by John D. Baldeschwieler, November 13, 1978. We acknowledge the encouragement and support of Prof. John D. Baldeschwieler. We also thank Drs. T. Y. Shen and M. M. Ponpipom (Merck, Sharp & Dohme Research Laboratories) for helpful discussions during this work. This investigation was supported by grants from the National Institutes of Health (GM 21111-06), the National Science Foundation (CHE 75-15146-A03), and Merck Sharp & Dohme. This is contribution no. 5891 from the Arthur Amos Noyes Laboratory of Chemical Physics. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.
Subject Keywords:liposomes; drug-delivery system; gamma-ray directional correlation
Issue or Number:2
Record Number:CaltechAUTHORS:MAUpnas79
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7707
Deposited By: Tony Diaz
Deposited On:02 Jul 2007
Last Modified:08 Nov 2021 20:45

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