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Structure of inserted bacteriophage Mu-1 DNA and physical mapping of bacterial genes by Mu-1 DNA insertion

Hsu, Ming-Ta and Davidson, Norman (1972) Structure of inserted bacteriophage Mu-1 DNA and physical mapping of bacterial genes by Mu-1 DNA insertion. Proceedings of the National Academy of Sciences of the United States of America, 69 (10). pp. 2823-2827. ISSN 0027-8424. doi:10.1073/pnas.69.10.2823.

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It is shown, by electron microscope observation of the structures of heteroduplexes, that Mu-1 DNA inserted into the bacterial episomes Flac and F8[1] is collinear with, rather than a circulation permutation of, the DNA of the mature Mu-1 bacteriophage. Observation of the position of the inserted Mu defines a point within the gene that has been inactivated (the lacI gene for Flac and a transfer gene in F8[1] in these particular instances). These examples illustrate a new, general method for physical gene mapping. The episome with Mu DNA inserted into F8[1] [i.e., F8[1](Mu)], although derived from a single colony, is heterogeneous in that a self-renatured sample shows a nonhomology loop of length 3.0 kb. This nonhomology loop, which has previously been observed in mature Mu-1 DNA, is due to an inversion.

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Additional Information:© 1972 by the National Academy of Sciences. Contributed by Norman Davidson, July 31, 1972. We are grateful to Ellen Daniell and Prof. John Abelson of the University of California at San Diego and to Prof. A. L. Taylor of the University of Colorado Medical School for educating us and stimulating our interest in the properties of Mu-1 bacteriophage DNA. The sample of Mu-1 DNA used in our studies was a generous gift from John Abelson and Ellen Daniell. The first observations in our laboratory of the heterogeneous structure of Mu DNA, as observed in self-renatured samples, were made by J. P. Kim, E. Daniell, and J. Abelson. We appreciate the guidance and advice of E. Ohtsubo throughout this work. This research has been supported by Grant GM 10991 from the NIH. This is Contribution No. 4502 from this Institute.
Subject Keywords:electron microscopy; episomes; Flac; E. coli
Issue or Number:10
Record Number:CaltechAUTHORS:HSUpnas72
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7709
Deposited By: Tony Diaz
Deposited On:02 Jul 2007
Last Modified:08 Nov 2021 20:45

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