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A cleavage map of bacteriophage Phi-X174 genome

Lee, Amy Shiu and Sinsheimer, Robert L. (1974) A cleavage map of bacteriophage Phi-X174 genome. Proceedings of the National Academy of Sciences of the United States of America, 71 (7). pp. 2882-2886. ISSN 0027-8424. PMCID PMC388576.

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Restriction endonucleases isolated from Hemophilus influenzae, Hemophilus parainfluenzae, and Hemophilus aegyptius were used to cleave phi-X174 replicative form DNA into three sets of specific DNA fragments. The order of these fragments in the phi-X replicative form molecule was determined by (1) analysis of partial digest products, (2) analysis of overlapping sets of fragments produced by two different restrictive enzymes. On the basis of these results, a detailed physical map of the phi-X174 genome has been constructed with respect to the cleavage sites of all three enzymes.

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Additional Information:© 1974 by the National Academy of Sciences. Contributed by Robert L. Sinsheimer, April 10, 1974. We thank Dr. C. Hutchinson, III for sending us the H. aegyptius strain, Dr. P.H. Johnson for the generous supply of [32P]phi-X RF, and Ms. Carole Latham for excellent technical assistance. This investigation was supported by the McCallum Fund of the Division of Biology of the California Institute of Technology and by USPHS Grant GM13554.
Funding AgencyGrant Number
Caltech McCallum FundUNSPECIFIED
U.S. Public Health Service (USPHS)GM 13554
Subject Keywords:restriction endonuclease; DNA fragments; DNA mapping; gel electrophoresis
Issue or Number:7
PubMed Central ID:PMC388576
Record Number:CaltechAUTHORS:LEEpnas74
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7728
Deposited By: Tony Diaz
Deposited On:30 Jul 2007
Last Modified:02 Oct 2019 23:44

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