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Ultrathin Coatings from Isocyanate Terminated Star PEG Prepolymers: Patterning of Proteins on the Layers

Groll, Juergen and Haubensak, Wulf and Ameringer, Thomas and Moeller, Martin (2005) Ultrathin Coatings from Isocyanate Terminated Star PEG Prepolymers: Patterning of Proteins on the Layers. Langmuir, 21 (7). pp. 3076-3083. ISSN 0743-7463. https://resolver.caltech.edu/CaltechAUTHORS:20170510-071217923

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Abstract

This study presents the easy and fast patterning of low molecular weight molecules that act as binding partners for proteins on Star PEG coatings. These coatings are prepared from isocyanate terminated star shaped prepolymers and form a highly cross-linked network on the substrate in which the stars are connected via urea groups and free amino groups are present. Streptavidin has been patterned on these layers by microcontact printing (μCP) of an amino reactive biotin derivative and consecutive binding of streptavidin to the biotin. Patterns of Ni^(2+)-nitriltriacetic acid (NTA) receptors have been prepared by printing amino functional NTA molecules in freshly prepared Star PEG layers that still contain amino reactive isocyanate groups. Complexation of the NTA groups with Ni(II) ions enabled the binding of His-tag enhanced green fluorescent protein (EGFP) in the desired pattern on the substrates. Since the unmodified Star PEG layers prevent unspecific protein adsorption, His-EGFP could selectively be bound to the sample by immersion into crude, nonpurified His-tag EGFP containing cell lysate.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/la047438nDOIArticle
http://pubs.acs.org/doi/abs/10.1021/la047438nPublisherArticle
Additional Information:© 2005 American Chemical Society. Received 18 October 2004. Published online 24 February 2005. Published in print 1 March 2005. The authors wish to thank Dr. David Drechsel and Jamie White for providing the purified His-tag EGFP and the His-tag EGFP expression vector, respectively. This work has been financially supported by the VolkswagenStiftung. The support of SusTech (Darmstadt, Germany) considering the Star PEG system is gratefully acknowledged.
Funders:
Funding AgencyGrant Number
VolkswagenStiftungUNSPECIFIED
SusTech (Darmstadt, Germany)UNSPECIFIED
Issue or Number:7
Record Number:CaltechAUTHORS:20170510-071217923
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170510-071217923
Official Citation:Ultrathin Coatings from Isocyanate Terminated Star PEG Prepolymers:  Patterning of Proteins on the Layers Juergen Groll, Wulf Haubensak, Thomas Ameringer, and Martin Moeller Langmuir 2005 21 (7), 3076-3083 DOI: 10.1021/la047438n
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:77324
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:16 May 2017 20:52
Last Modified:03 Oct 2019 17:56

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