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Tertiary Contact Formation in α-Synuclein Probed by Electron Transfer

Lee, Jennifer C. and Gray, Harry B. and Winkler, Jay R. (2005) Tertiary Contact Formation in α-Synuclein Probed by Electron Transfer. Journal of the American Chemical Society, 127 (47). pp. 16388-16389. ISSN 0002-7863. doi:10.1021/ja0561901.

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To explore tertiary contact formation in α-synuclein, a natively unfolded protein implicated in Parkinson's disease, we have measured the rates of reaction between a powerful electron donor, the tryptophan (W) triplet excited state, and an acceptor, 3-nitro-tyrosine (Y(NO_2)) in six different variants, probing loop sizes between 15 and 132 residues. Electron transfer rates decrease with loop size with the fastest contact time of 140 ns for the N-terminal pair and the slowest of 1.2 μs for the N- to C-terminal pair. Diffusion coefficients ranging from ∼2 × 10^(-6) to ∼10^(-5) cm^2 s^(-1) were extracted from simultaneous fits of the W to Y(NO_2) electron (triplet excited state) and energy transfer (singlet excited state) kinetics.

Item Type:Article
Related URLs:
URLURL TypeDescription Information
Gray, Harry B.0000-0002-7937-7876
Winkler, Jay R.0000-0002-4453-9716
Additional Information:© 2005 American Chemical Society. Received September 8, 2005. Publication Date (Web): November 3, 2005. Supported by the Parkinson's Disease Foundation (J.R.W.), the National Parkinson Foundation (J.R.W.), NIH (GM068461 to J.R.W.), DOE (DE-FG02-02ER15359 to J.R.W.), the Arnold and Mabel Beckman Foundation (Beckman Senior Research Fellowship to J.C.L.), the Beckman Macular Research Center (H.B.G.), and the Ellison Medical Foundation (Senior Scholar Award in Aging to H.B.G.).
Funding AgencyGrant Number
Parkinson's Disease FoundationUNSPECIFIED
National Parkinson FoundationUNSPECIFIED
Department of Energy (DOE)DE-FG02-02ER15359
Arnold and Mabel Beckman FoundationUNSPECIFIED
Beckman Macular Research CenterUNSPECIFIED
Ellison Medical FoundationUNSPECIFIED
Issue or Number:47
Record Number:CaltechAUTHORS:20170512-124549828
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Official Citation:Tertiary Contact Formation in α-Synuclein Probed by Electron Transfer Jennifer C. Lee, Harry B. Gray, and Jay R. Winkler Journal of the American Chemical Society 2005 127 (47), 16388-16389 DOI: 10.1021/ja0561901
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:77415
Deposited By: Tony Diaz
Deposited On:12 May 2017 23:31
Last Modified:15 Nov 2021 17:30

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