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Nature of the Cytochrome c Molten Globule

Pletneva, Ekaterina V. and Gray, Harry B. and Winkler, Jay R. (2005) Nature of the Cytochrome c Molten Globule. Journal of the American Chemical Society, 127 (44). pp. 15370-15371. ISSN 0002-7863. doi:10.1021/ja0555318. https://resolver.caltech.edu/CaltechAUTHORS:20170516-102143640

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Abstract

We have employed fluorescence energy transfer (FET) kinetics to probe unfolded and molten globule states of five dansyl (Dns) variants of Saccharomyces cerevisiae iso-1 cytochrome c. The covalently bound Fe(III) heme group quenches Dns fluorescence by energy transfer; measurements of FET kinetics yielded distributions of D−A distances (P(r)) for these states. The P(r) distributions and corresponding mean force potentials (U(r)) show that the cytochrome c molten globule is a highly structured state with a substantial number of native interactions. Wide P(r) distributions directly reflect the dynamic nature and conformational diversity of this molten globule. P(r) distributions for the “burst-phase” refolding intermediate suggest that the equilibrium cytochrome c molten globule is not a suitable model for early intermediates formed during protein refolding.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja0555318DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja0555318PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja0555318PublisherSupporting Information
ORCID:
AuthorORCID
Gray, Harry B.0000-0002-7937-7876
Winkler, Jay R.0000-0002-4453-9716
Additional Information:© 2005 American Chemical Society. Received 12 August 2005. Published online 13 October 2005. Published in print 1 November 2005. This work was supported by NIH GM068461 (J.R.W.) and the Ellison Medical Foundation (Senior Scholar Award in Aging to H.B.G.).
Funders:
Funding AgencyGrant Number
NIHGM068461
Ellison Medical FoundationUNSPECIFIED
Issue or Number:44
DOI:10.1021/ja0555318
Record Number:CaltechAUTHORS:20170516-102143640
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170516-102143640
Official Citation:Nature of the Cytochrome c Molten Globule Ekaterina V. Pletneva, Harry B. Gray, and Jay R. Winkler Journal of the American Chemical Society 2005 127 (44), 15370-15371 DOI: 10.1021/ja0555318
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:77492
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:16 May 2017 19:15
Last Modified:15 Nov 2021 17:31

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