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Nature of the Cytochrome c Molten Globule

Pletneva, Ekaterina V. and Gray, Harry B. and Winkler, Jay R. (2005) Nature of the Cytochrome c Molten Globule. Journal of the American Chemical Society, 127 (44). pp. 15370-15371. ISSN 0002-7863. doi:10.1021/ja0555318.

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We have employed fluorescence energy transfer (FET) kinetics to probe unfolded and molten globule states of five dansyl (Dns) variants of Saccharomyces cerevisiae iso-1 cytochrome c. The covalently bound Fe(III) heme group quenches Dns fluorescence by energy transfer; measurements of FET kinetics yielded distributions of D−A distances (P(r)) for these states. The P(r) distributions and corresponding mean force potentials (U(r)) show that the cytochrome c molten globule is a highly structured state with a substantial number of native interactions. Wide P(r) distributions directly reflect the dynamic nature and conformational diversity of this molten globule. P(r) distributions for the “burst-phase” refolding intermediate suggest that the equilibrium cytochrome c molten globule is not a suitable model for early intermediates formed during protein refolding.

Item Type:Article
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URLURL TypeDescription Information
Gray, Harry B.0000-0002-7937-7876
Winkler, Jay R.0000-0002-4453-9716
Additional Information:© 2005 American Chemical Society. Received 12 August 2005. Published online 13 October 2005. Published in print 1 November 2005. This work was supported by NIH GM068461 (J.R.W.) and the Ellison Medical Foundation (Senior Scholar Award in Aging to H.B.G.).
Funding AgencyGrant Number
Ellison Medical FoundationUNSPECIFIED
Issue or Number:44
Record Number:CaltechAUTHORS:20170516-102143640
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Official Citation:Nature of the Cytochrome c Molten Globule Ekaterina V. Pletneva, Harry B. Gray, and Jay R. Winkler Journal of the American Chemical Society 2005 127 (44), 15370-15371 DOI: 10.1021/ja0555318
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:77492
Deposited By: Ruth Sustaita
Deposited On:16 May 2017 19:15
Last Modified:15 Nov 2021 17:31

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