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Charting the protein complexome in yeast by mass spectrometry

Deshaies, Raymond J. and Seol, Jae Hong and McDonald, W. Hayes and Cope, Greg and Lyapina, Svetlana and Shevchenko, Andrej and Shevchenko, Anna and Verma, Rati and Yates, John R., III (2002) Charting the protein complexome in yeast by mass spectrometry. Molecular and Cellular Proteomics, 1 (1). pp. 3-10. ISSN 1535-9476. http://resolver.caltech.edu/CaltechAUTHORS:DESmcp02

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Abstract

It has become evident over the past few years that many complex cellular processes, including control of the cell cycle and ubiquitin-dependent proteolysis, are carried out by sophisticated multisubunit protein machines that are dynamic in abundance, post-translational modification state, and composition. To understand better the nature of the macromolecular assemblages that carry out the cell cycle and ubiquitin-dependent proteolysis, we have used mass spectrometry extensively over the past few years to characterize both the composition of various protein complexes and the modification states of their subunits. In this article we review some of our recent efforts, and describe a promising new approach for using mass spectrometry to dissect protein interaction networks.


Item Type:Article
ORCID:
AuthorORCID
Deshaies, Raymond J.0000-0002-3671-9354
Additional Information:© 2002 by The American Society for Biochemistry and Molecular Biology, Inc. Received, October 9, 2001, and in revised form, November 19, 2001. Published, MCP Papers in Press, November 21, 2001, DOI 10.1074/mcp.R100001-MCP200 The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Subject Keywords:UBIQUITIN-LIGASE COMPLEX; CYCLIN-DEPENDENT-KINASE; S-PHASE; TRANSCRIPTION FACTORS; COP9 SIGNALOSOME; FISSION YEAST; F-BOX; DEGRADATION; SCF; ARABIDOPSIS
Issue or Number:1
Record Number:CaltechAUTHORS:DESmcp02
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:DESmcp02
Alternative URL:http://dx.doi.org/10.1074/mcp.R100001-MCP200
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7752
Collection:CaltechAUTHORS
Deposited By: Lindsay Cleary
Deposited On:30 Jul 2007
Last Modified:07 Nov 2017 21:53

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