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An integrated LC-ESI chip with electrochemical-based gradient generation

Xie, Jun and Shih, Jason and Pang, Changlin and Tai, Yu-Chong and Miao, Yunan and Lee, Terry D. (2004) An integrated LC-ESI chip with electrochemical-based gradient generation. In: 17th IEEE International Conference on Micro Electro Mechanical Systems. Maastricht MEMS 2004 Technical Digest. IEEE , Piscataway, NJ, pp. 334-337. ISBN 0-7803-8265-X. https://resolver.caltech.edu/CaltechAUTHORS:20170518-111051244

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Abstract

This work reports the first on-chip generation of gradient elution using electrochemical pumping, for application in Liquid Chromatography-Electrospray Ionization (LC-ESI). The system consists of Parylene, SU-8 and PDMS parts to create two 3 μL on-chip solvent reservoirs, two electrochemical pumps and an ESI nozzle. Pumping is controlled galvanostatically (i.e., through electrolysis current). At low back pressures, pumping rate can reach 200 nL/min using just 40 μA (power consumption <100 μW). At 80 psi, 200 μA (power consumption <1 mW) is sufficient to deliver fluid at 75 nL/min. Higher electrical currents have been used to increase the flow rate or to achieve pumping at higher back pressures. For example, pumping at 200 psi back pressure has been demonstrated using a current of 800 μA. Electrically controlled on-chip gradient generation is achieved without any external fluidic connections to the chip. Continuous generation of gradients for 30 minutes at a total flow rate of ~100 nL/min is also realized and confirmed with mass spectroscopy (MS) measurements. The developed on-chip gradient generation system meets the general requirements of LC applications.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1109/MEMS.2004.1290590DOIArticle
http://ieeexplore.ieee.org/document/1290590/PublisherArticle
ORCID:
AuthorORCID
Tai, Yu-Chong0000-0001-8529-106X
Additional Information:© 2004 IEEE. This work is supported in part by NSF ERC Center at CALTECH (EEC-9402726), NIH (5R01 RR06217-10), and DARPA Bioflips Program (N66001-00-C-8092).
Funders:
Funding AgencyGrant Number
NSFEEC-9402726
NIH5R01 RR06217-10
Defense Advanced Research Projects Agency (DARPA)N66001-00-C-8092
Record Number:CaltechAUTHORS:20170518-111051244
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170518-111051244
Official Citation:Jun Xie, Jason Shih, Changlin Pang, Yu-Chong Tai, Yunan Miao and T. D. Lee, "An integrated LC-ESI chip with electrochemical-based gradient generation," 17th IEEE International Conference on Micro Electro Mechanical Systems. Maastricht MEMS 2004 Technical Digest, 2004, pp. 334-337. doi: 10.1109/MEMS.2004.1290590
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:77571
Collection:CaltechAUTHORS
Deposited By: Kristin Buxton
Deposited On:18 May 2017 18:45
Last Modified:03 Oct 2019 17:59

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