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Extraction and Measurement of NAD(P)+ and NAD(P)H

Kern, Suzanne E. and Price-Whelan, Alexa and Newman, Dianne K. (2014) Extraction and Measurement of NAD(P)+ and NAD(P)H. In: Pseudomonas Methods and Protocols. Methods in Molecular Biology. No.1149. Humana Press , New York, NY, pp. 311-323. ISBN 9781493904723.

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Nicotinamide adenine dinucleotides are critical redox-active substrates for countless catabolic and anabolic reactions. Ratios of NAD+ to NADH and NADP+ to NADPH are therefore considered key indicators of the overall intracellular redox potential and metabolic state. These ratios can be measured in bulk conditions using a highly sensitive enzyme cycling-based colorimetric assay (detection limit at or below 0.05 μM or 1 pmol) following a simple extraction procedure involving solutions of acid and base. Special considerations are necessary to avoid measurement artifacts caused by the presence of endogenous redox-active metabolites, such as phenazines made by diverse Pseudomonas species (see Chapter 25).

Item Type:Book Section
Related URLs:
URLURL TypeDescription Chapter Chapter
Price-Whelan, Alexa0000-0003-0872-7098
Newman, Dianne K.0000-0003-1647-1918
Additional Information:Ⓒ 2014 Springer Science+Business Media New York.
Series Name:Methods in Molecular Biology
Issue or Number:1149
Record Number:CaltechAUTHORS:20170518-165224584
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:77593
Deposited By: Donna Wrublewski
Deposited On:19 May 2017 15:03
Last Modified:15 Nov 2021 17:32

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