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Measurement of Phenazines in Bacterial Cultures

Kern, Suzanne E. and Newman, Dianne K. (2014) Measurement of Phenazines in Bacterial Cultures. In: Pseudomonas Methods and Protocols. Methods in Molecular Biology. No.1149. Springer , New York, pp. 303-310. ISBN 9781493904723.

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Certain pseudomonads are capable of producing phenazines—pigmented, reversibly redox-active metabolites that induce a variety of physiological effects on the producing organism as well as others in their vicinity. Environmental conditions and the specific physiological state of cells can dramatically affect the absolute amounts and relative proportions of the various phenazines produced. The method detailed here—high-performance liquid chromatography coupled to detection by UV–Vis absorption—can be used to separate and quantify the amount of phenazines in a Pseudomonas culture. Simple spectrophotometric measurements of filtered culture supernatants can be used to quantify certain oxidized phenazines, such as pyocyanin, in cultures. For cases where the conditions under study are not planktonic cultures (e.g., soil or biofilms) extracting the phenazines may be a necessary first step.

Item Type:Book Section
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URLURL TypeDescription Chapter Chapter
Newman, Dianne K.0000-0003-1647-1918
Additional Information:Ⓒ 2014 Springer Science+Business Media New York.
Subject Keywords:Phenazine; Pyocyanin; Phenazine-1-carboxylic acid; 1-Hydroxyphenazine; PCA; PYO; HPLC; UV–Vis spectroscopy
Series Name:Methods in Molecular Biology
Issue or Number:1149
Record Number:CaltechAUTHORS:20170522-090349682
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:77621
Deposited By: Donna Wrublewski
Deposited On:22 May 2017 16:42
Last Modified:15 Nov 2021 17:32

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