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Global Representations of Goal-Directed Behavior in Distinct Cell Types of Mouse Neocortex

Allen, William E. and Kauvar, Isaac V. and Chen, Michael Z. and Richman, Ethan B. and Yang, Samuel J. and Chan, Ken and Gradinaru, Viviana and Deverman, Benjamin E. and Luo, Liqun and Deisseroth, Karl (2017) Global Representations of Goal-Directed Behavior in Distinct Cell Types of Mouse Neocortex. Neuron, 94 (4). pp. 891-907. ISSN 0896-6273. PMCID PMC5723385. https://resolver.caltech.edu/CaltechAUTHORS:20170522-103736207

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Abstract

The successful planning and execution of adaptive behaviors in mammals may require long-range coordination of neural networks throughout cerebral cortex. The neuronal implementation of signals that could orchestrate cortex-wide activity remains unclear. Here, we develop and apply methods for cortex-wide Ca^(2+) imaging in mice performing decision-making behavior and identify a global cortical representation of task engagement encoded in the activity dynamics of both single cells and superficial neuropil distributed across the majority of dorsal cortex. The activity of multiple molecularly defined cell types was found to reflect this representation with type-specific dynamics. Focal optogenetic inhibition tiled across cortex revealed a crucial role for frontal cortex in triggering this cortex-wide phenomenon; local inhibition of this region blocked both the cortex-wide response to task-initiating cues and the voluntary behavior. These findings reveal cell-type-specific processes in cortex for globally representing goal-directed behavior and identify a major cortical node that gates the global broadcast of task-related information.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.neuron.2017.04.017DOIArticle
http://www.sciencedirect.com/science/article/pii/S0896627317303434PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5723385PubMed CentralArticle
ORCID:
AuthorORCID
Gradinaru, Viviana0000-0001-5868-348X
Deverman, Benjamin E.0000-0002-6223-9303
Additional Information:© 2017 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Open Access funded by the US Department of Defense (DoD) or performed by an employee of DoD. Received 17 June 2016, Revised 26 January 2017, Accepted 11 April 2017, Available online 17 May 2017. We would like to thank C. Ramakrishnan for molecular biology assistance and for cloning the CAG-DIO-GCaMP6f construct, J. Fernandez-Alcudia (Stanford Gene Vector and Virus Core) for producing AAV-PHP.B, H. Zeng (Allen Institute for Brain Science) for providing transgenic mice prior to publication, J. Sanders (Cold Spring Harbor Laboratory) for providing Bpod code and hardware designs, T. Komiyama (University of California, San Diego) for providing initial olfactometer advice, N. Young for image acquisition code, Z. Guo and K. Svoboda (Janelia Research Campus) for advice on the surgical preparation, and C. Manalac for assistance with genotyping. We are grateful to members of the K.D. and L.L. laboratories for comments and advice, particularly M. Lovett-Barron, P. Rajasethupathy, C. Kim, and A. Andalman. V.G. is a Heritage Principal Investigator supported by the Heritage Medical Research Institute. AAV-PHP.B work was funded by NIH grant DP2/PECASE (V.G.) and by the Beckman Institute at Caltech (V.G. and B.E.D.) through the Resource Center on CLARITY, Optogenetics, and Vector Engineering. K.D. and L.L. are Investigators of the Howard Hughes Medical Institute and are supported by a Hughes Collaborative Innovation Award. W.E.A. and I.V.K. were supported by National Science Foundation Graduate Research Fellowships (grant DGE-114747) and W.E.A. by a Fannie & John Hertz Foundation Fellowship. K.D. is supported by the Defense Advanced Research Projects Agency Neuro-FAST program, the National Institute of Mental Health, the National Institute on Drug Abuse, the National Science Foundation, the NOMIS Foundation, the Tarlton Foundation, the Wiegers Family Fund, the Nancy and James Grosfeld Foundation, the H.L. Snyder Medical Foundation, and the Samuel and Betsy Reeves Fund. All tools and methods are distributed and supported freely.
Group:Heritage Medical Research Institute
Funders:
Funding AgencyGrant Number
Heritage Medical Research InstituteUNSPECIFIED
NIHDP2/PECASE
Caltech Beckman InstituteUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
NSF Graduate Research FellowshipDGE-114747
Fannie and John Hertz FoundationUNSPECIFIED
Defense Advanced Research Projects Agency (DARPA)UNSPECIFIED
National Institute of Mental Health (NIMH)UNSPECIFIED
National Institute on Drug AbuseUNSPECIFIED
NSFUNSPECIFIED
NOMIS FoundationUNSPECIFIED
Tarlton FoundationUNSPECIFIED
Wiegers Family FundUNSPECIFIED
Nancy and James Grosfeld FoundationUNSPECIFIED
H. L. Snyder Medical FoundationUNSPECIFIED
Samuel and Betsy Reeves FundUNSPECIFIED
Department of DefenseUNSPECIFIED
Subject Keywords:cortex; goal-directed behavior; calcium imaging; widefield; cell type; optogenetics
Issue or Number:4
PubMed Central ID:PMC5723385
Record Number:CaltechAUTHORS:20170522-103736207
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170522-103736207
Official Citation:William E. Allen, Isaac V. Kauvar, Michael Z. Chen, Ethan B. Richman, Samuel J. Yang, Ken Chan, Viviana Gradinaru, Benjamin E. Deverman, Liqun Luo, Karl Deisseroth, Global Representations of Goal-Directed Behavior in Distinct Cell Types of Mouse Neocortex, Neuron, Volume 94, Issue 4, 17 May 2017, Pages 891-907.e6, ISSN 0896-6273, https://doi.org/10.1016/j.neuron.2017.04.017. (http://www.sciencedirect.com/science/article/pii/S0896627317303434)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:77626
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:22 May 2017 18:31
Last Modified:03 Oct 2019 17:59

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