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A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates

Lee, Juhyun and Chou, Tzu-Chieh and Kang, Dongyang and Kang, Hanul and Chen, Junjie and Baek, Kyung In and Wang, Wei and Ding, Yichen and Carlo, Dino Di and Tai, Yu-Chong and Hsiai, Tzung K. (2017) A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates. Scientific Reports, 7 . Art. No. 1980. ISSN 2045-2322. PMCID PMC5434032. https://resolver.caltech.edu/CaltechAUTHORS:20170522-145005144

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Abstract

Blood viscosity provides the rheological basis to elucidate shear stress underlying developmental cardiac mechanics and physiology. Zebrafish is a high throughput model for developmental biology, forward-genetics, and drug discovery. The micro-scale posed an experimental challenge to measure blood viscosity. To address this challenge, a microfluidic viscometer driven by surface tension was developed to reduce the sample volume required (3μL) for rapid (<2 min) and continuous viscosity measurement. By fitting the power-law fluid model to the travel distance of blood through the micro-channel as a function of time and channel configuration, the experimentally acquired blood viscosity was compared with a vacuum-driven capillary viscometer at high shear rates (>500 s^(−1)), at which the power law exponent (n) of zebrafish blood was nearly 1 behaving as a Newtonian fluid. The measured values of whole blood from the micro-channel (4.17cP) and the vacuum method (4.22cP) at 500 s^(−1) were closely correlated at 27 °C. A calibration curve was established for viscosity as a function of hematocrits to predict a rise and fall in viscosity during embryonic development. Thus, our rapid capillary pressure-driven micro-channel revealed the Newtonian fluid behavior of zebrafish blood at high shear rates and the dynamic viscosity during development.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/s41598-017-02253-7DOIArticle
http://www.nature.com/articles/s41598-017-02253-7PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5434032/PubMed CentralArticle
ORCID:
AuthorORCID
Chou, Tzu-Chieh0000-0002-6074-8286
Ding, Yichen0000-0002-6242-3506
Tai, Yu-Chong0000-0001-8529-106X
Additional Information:© 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Received: 06 December 2016; Accepted: 07 April 2017; Published online: 16 May 2017. This study was supported by the National Institutes of Health (NIH) HL118650 (T.K.H., Y.C.T.), HL083015 (T.K.H.), HL111437 (T.K.H., Y.C.T.), HL129727 (T.K.H.), and American Heart Association (AHA) Pre-Doctoral Fellowship 15PRE21400019 (J.L.). Author Contributions: J.L., T.C., D.K., and W.W. designed experiment plan. J.L., H.K., J.C., and K.I.B. performed experiments. T.C., D.K., and W.W. fabricated microfluidic channels. J.C., and Y.D. performed hematocrit from embryonic zebrafish. D.C., Y.T., and T.K.H. supervised and supported the study. The authors declare that they have no competing interests.
Funders:
Funding AgencyGrant Number
NIHHL118650
NIHHL083015
NIHHL111437
NIHHL129727
American Heart Association15PRE21400019
PubMed Central ID:PMC5434032
Record Number:CaltechAUTHORS:20170522-145005144
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170522-145005144
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:77632
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:22 May 2017 22:47
Last Modified:03 Oct 2019 18:00

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