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Diversity and structure of human T-Cell receptor β-chain variable region genes

Concannon, Patrick and Pickering, Lulu A. and Kung, Patrick and Hood, Leroy (1986) Diversity and structure of human T-Cell receptor β-chain variable region genes. Proceedings of the National Academy of Sciences of the United States of America, 83 (17). pp. 6598-6602. ISSN 0027-8424. PMCID PMC386551. doi:10.1073/pnas.83.17.6598.

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The nucleotide sequences of 27 T-cell receptor β cDNA clones isolated from a human peripheral lymphocyte library were determined and compared to five additional published sequences. These cDNA clones represent 22 distinct T-cell receptor β-chain variable region (Vβ) gene segment sequences, which fall into 15 different Vβ gene subfamilies, each containing six or fewer members. From this analysis, we estimate that the repertoire of expressed human Vβ genes is <59, apparently much smaller than the immunoglobulin heavy chain and light chain variable region (VH and VL) repertoires. Variability plots comparing these human Vβ regions with each other and with published mouse Vβ regions provide evidence for only four hypervariable regions homologous to those seen in comparisons of immunoglobulin V regions. Somatic hypermutation appears to be used infrequently, if at all, in these Vβ genes.

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Hood, Leroy0000-0001-7158-3678
Additional Information:© 1986 by the National Academy of Sciences. Contributed by Leroy Hood, May 13, 1986. We thank Dr. Suzanna Horvath for synthesis of oligonucleotide sequencing primers; Drs. Howard Gershenfeld and Stephen Crews for cDNA protocols and T-cell RNA; Dr. Richard Gatti for phytohemagglutinin-stimulated human peripheral lymphocytes; Dennis Mock for the statistical estimation of Vβ repertoire size; Jocyndra Wright of T Cell Sciences, Inc., for assistance in DNA sequencing; Dr. William Gilbert for help in modifying DNA sequence analysis programs; Mr. Tim Hunkapiller for assistance in sequence alignment and analysis; and Connie Katz, Susan Mangrum, and Cathy Elkins for excellent manuscript preparation. This work was supported in part by a grant from the National Institutes of Health. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
Subject Keywords:nucleotide sequence; repertoire size; gene subfamilies; somatic mutation
Issue or Number:17
PubMed Central ID:PMC386551
Record Number:CaltechAUTHORS:CONpnas86a
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7764
Deposited By: Tony Diaz
Deposited On:31 Jul 2007
Last Modified:08 Nov 2021 20:45

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