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α-Synuclein Tertiary Contact Dynamics

Lee, Jennifer C. and Lai, Bert T. and Kozak, John J. and Gray, Harry B. and Winkler, Jay R. (2007) α-Synuclein Tertiary Contact Dynamics. Journal of Physical Chemistry B, 111 (8). pp. 2107-2112. ISSN 1520-6106. PMCID PMC2519050. doi:10.1021/jp068604y.

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Tertiary contact formation rates in α-synuclein, an intrinsically disordered polypeptide implicated in Parkinson's disease, have been determined from measurements of diffusion-limited electron-transfer kinetics between triplet-excited tryptophan:3-nitrotyrosine pairs separated by 10, 12, 55, and 90 residues. Calculations based on a Markovian lattice model developed to describe intrachain diffusion dynamics for a disordered polypeptide give contact quenching rates for various loop sizes ranging from 6 to 48 that are in reasonable agreement with experimentally determined values for small loops (10−20 residues). Contrary to expectations, measured contact rates in α-synuclein do not continue to decrease as the loop size increases (≥35 residues), and substantial deviations from calculated rates are found for the pairs W4−Y94, Y39−W94, and W4−Y136. The contact rates for these large loops indicate much shorter average donor−acceptor separations than expected for a random polymer.

Item Type:Article
Related URLs:
URLURL TypeDescription Information CentralArticle
Gray, Harry B.0000-0002-7937-7876
Winkler, Jay R.0000-0002-4453-9716
Additional Information:© 2007 American Chemical Society. Received: December 14, 2006. Publication Date (Web): February 6, 2007. This work was supported by the National Institutes of Health (GM068461 to J.R.W.; DK19038 to H.B.G.), the Arnold and Mabel Beckman Foundation (Beckman Senior Research Fellowship to J.C.L.), and the Ellison Medical Foundation (Senior Scholar Award in Aging to H.B.G.).
Funding AgencyGrant Number
Arnold and Mabel Beckman FoundationUNSPECIFIED
Ellison Medical FoundationUNSPECIFIED
Issue or Number:8
PubMed Central ID:PMC2519050
Record Number:CaltechAUTHORS:20170524-101700076
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Official Citation:α-Synuclein Tertiary Contact Dynamics Jennifer C. Lee, Bert T. Lai, John J. Kozak, Harry B. Gray, and Jay R. Winkler The Journal of Physical Chemistry B 2007 111 (8), 2107-2112 DOI: 10.1021/jp068604y
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:77706
Deposited By: Tony Diaz
Deposited On:24 May 2017 18:28
Last Modified:15 Nov 2021 17:33

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