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Black Lipid Membranes: Visualizing the Structure, Dynamics, and Substrate Dependence of Membranes

Ries, Ryan S. and Choi, Hyeon and Blunck, Rikard and Bezanilla, Francisco and Heath, James R. (2004) Black Lipid Membranes: Visualizing the Structure, Dynamics, and Substrate Dependence of Membranes. Journal of Physical Chemistry B, 108 (41). pp. 16040-16049. ISSN 1520-6106. https://resolver.caltech.edu/CaltechAUTHORS:20170524-141911014

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Abstract

The structure and long-time dynamics of suspended lipid bilayers made of 1-stearoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (SOPE) in the gel phase was interrogated using electrical measurements co-recorded with second harmonic generation (SHG) micrographs. SHG microscopy was shown to be an efficient method for distinguishing between the regions of bilayer and annulus as well as probing structural and dynamical variations within a single bilayer. Micromachined silicon chips and two types of plastic partitions, delrin and polyethylene, were investigated as substrate materials. Silicon chips yielded the most stable bilayers, lasting for 1 or 2 days. The membrane characteristics, including the amount of incorporated solvent within the bilayer, the dynamics of bilayer formation, and the stability of the bilayer, were found to be strongly substrate-dependent. In a second set of experiments, SHG-active membrane dyes, di-8-ANEPPS and di-4-ANEPPS, were used to interrogate the domain structure of the same suspended bilayers using scanning two-photon fluorescence (2PF) and SHG microscopy, again correlated with capacitance measurements. Using these dyes, an enhancement in the SHG signal due to membrane potential was recorded, and the rates of molecular diffusion for di-4-ANEPPS through the membrane were investigated. Polarization-resolved SHG imaging was used to determine the orientation of the dye molecules within the membrane.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/jp048098hDOIArticle
http://pubs.acs.org/doi/abs/10.1021/jp048098hPublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/jp048098hPublisherSupporting Information
ORCID:
AuthorORCID
Blunck, Rikard0000-0003-4484-2907
Heath, James R.0000-0001-5356-4385
Additional Information:© 2004 American Chemical Society. Received 3 May 2004. Published online 2 July 2004. Published in print 1 October 2004. We acknowledge the W.M. Keck Foundation for assistance in constructing the SHG and 2PF microscopes used in this work. R.B. and F.B. acknowledge support from the National Institutes of Health (USPHS GM30376). J.R.H., R.S.R, and H.C. acknowledge the Institute for Collaborative Biotechnologies through grant DAAD19-03-D-0004 from the U.S. Army Research Office. We thank Dr. Leslie Loew for his helpful conversations and insight regarding the dyes.
Funders:
Funding AgencyGrant Number
W. M. Keck FoundationUNSPECIFIED
NIHGM30376
Army Research Office (ARO)DAAD19-03-D-0004
Issue or Number:41
Record Number:CaltechAUTHORS:20170524-141911014
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170524-141911014
Official Citation:Black Lipid Membranes:  Visualizing the Structure, Dynamics, and Substrate Dependence of Membranes Ryan S. Ries, Hyeon Choi, Rikard Blunck, Francisco Bezanilla, and James R. Heath The Journal of Physical Chemistry B 2004 108 (41), 16040-16049 DOI: 10.1021/jp048098h
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:77725
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:24 May 2017 21:37
Last Modified:03 Oct 2019 18:00

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