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Electrochemistry of Cytochrome P450 BM3 in Sodium Dodecyl Sulfate Films

Udit, Andrew K. and Hill, Michael G. and Gray, Harry B. (2006) Electrochemistry of Cytochrome P450 BM3 in Sodium Dodecyl Sulfate Films. Langmuir, 22 (25). pp. 10854-10857. ISSN 0743-7463. PMCID PMC2515932. https://resolver.caltech.edu/CaltechAUTHORS:20170530-133240937

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Abstract

Direct electrochemistry of the cytochrome P450 BM3 heme domain (BM3) was achieved by confining the protein within sodium dodecyl sulfate (SDS) films on the surface of basal-plane graphite (BPG) electrodes. Cyclic voltammetry revealed the heme Fe^(III/II) redox couple at −330 mV (vs Ag/AgCl, pH 7.4). Up to 10 V/s, the peak current was linear with the scan rate, allowing us to treat the system as surface-confined within this regime. The standard heterogeneous rate constant determined at 10 V/s was estimated to be 10 s^(-1). Voltammograms obtained for the BM3−SDS−BPG system in the presence of dioxygen exhibited catalytic waves at the onset of Fe^(III) reduction. The altered heme reduction potential of the BM3−SDS−graphite system indicates that SDS is likely bound in the enzyme active-site region. Compared to other P450-surfactant systems, we find redox potentials and electron-transfer rates that differ by ∼100 mV and >10-fold, respectively, indicating that the nature of the surfactant environment has a significant effect on the observed heme redox properties.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/la061162xDOIArticle
http://pubs.acs.org/doi/abs/10.1021/la061162xPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2515932/PubMed CentralArticle
ORCID:
AuthorORCID
Gray, Harry B.0000-0002-7937-7876
Additional Information:© 2006 American Chemical Society. Received April 28, 2006. In Final Form: July 25, 2006. Publication Date (Web): September 2, 2006. We thank C. E. Immoos for helpful discussions and P. C. Cirino for providing BM3 mutant 5H6. We acknowledge support from NIH (H.B.G.), NSERC (A.K.U.), HHMI (A.K.U.), and the Ellison Medical Foundation (Senior Scholar Award in Aging to H.B.G.).
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Ellison Medical FoundationUNSPECIFIED
Issue or Number:25
PubMed Central ID:PMC2515932
Record Number:CaltechAUTHORS:20170530-133240937
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170530-133240937
Official Citation:Electrochemistry of Cytochrome P450 BM3 in Sodium Dodecyl Sulfate Films Andrew K. Udit, Michael G. Hill, and Harry B. Gray Langmuir 2006 22 (25), 10854-10857 DOI: 10.1021/la061162x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:77833
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:30 May 2017 20:47
Last Modified:22 Nov 2019 09:58

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