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Probing the cytochrome c′ folding landscape

Pletneva, Ekaterina V. and Zhao, Ziqing and Kimura, Tetsunari and Petrova, Krastina V. and Gray, Harry B. and Winkler, Jay R. (2007) Probing the cytochrome c′ folding landscape. Journal of Inorganic Biochemistry, 101 (11-12). pp. 1768-1775. ISSN 0162-0134. PMCID PMC2110879. doi:10.1016/j.jinorgbio.2007.06.019.

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The folding kinetics of R. palustris cytochrome c′ (cyt c′) have been monitored by heme absorption and native Trp72 fluorescence at pH 5. The Trp72 fluorescence burst signal suggests early compaction of the polypeptide ensemble. Analysis of heme transient absorption spectra reveals deviations from two-state behavior, including a prominent slow phase that is accelerated by the prolyl isomerase cyclophilin. A nonnative proline configuration (Pro21) likely interferes with the formation of the helical bundle surrounding the heme.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Gray, Harry B.0000-0002-7937-7876
Winkler, Jay R.0000-0002-4453-9716
Additional Information:© 2007 Elsevier Inc. Received 3 April 2007; received in revised form 31 May 2007; accepted 1 June 2007; Available online 21 June 2007. We thank Professor Amy H. Andreotti for providing human cyclophilin plasmid and Binghai Ling for screening bacterial colonies for cyt c′ expression. Our work was supported by NIH (GM068461 to J.R.W.), the Ellison Medical Foundation (Senior Scholar Award in Aging to H.B.G.), the Caltech SURF program (Mr. and Mrs. Samuel Krown fellowship to Z.Z.), and the Japan Society for the Promotion of Science for Young Scientists (Research Fellowship to T.K.).
Funding AgencyGrant Number
Ellison Medical FoundationUNSPECIFIED
Caltech Summer Undergraduate Research Fellowship (SURF)UNSPECIFIED
Japan Society for the Promotion of Science (JSPS)UNSPECIFIED
Subject Keywords:Folding; Kinetics; Intermediates; Singular-value decomposition
Issue or Number:11-12
PubMed Central ID:PMC2110879
Record Number:CaltechAUTHORS:20170531-085209969
Persistent URL:
Official Citation:Ekaterina V. Pletneva, Ziqing Zhao, Tetsunari Kimura, Krastina V. Petrova, Harry B. Gray, Jay R. Winkler, Probing the cytochrome ′ folding landscape, Journal of Inorganic Biochemistry, Volume 101, Issue 11, 2007, Pages 1768-1775, ISSN 0162-0134, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:77845
Deposited By: Tony Diaz
Deposited On:31 May 2017 18:50
Last Modified:15 Nov 2021 17:34

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