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Cytoskeleton dynamics in drug-treated platelets

Finkenstaedt-Quinn, Solaire A. and Ge, Shencheng and Haynes, Christy L. (2015) Cytoskeleton dynamics in drug-treated platelets. Analytical and Bioanalytical Chemistry, 407 (10). pp. 2803-2809. ISSN 1618-2642. PMCID PMC4370791.

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Platelet activation is a key process in blood clot formation. During activation, platelets go through both chemical and physical changes, including secretion of chemical messengers and cellular shape change. Platelet shape change is mediated by the two major cytoskeletal elements in platelets, the actin matrix and microtubule ring. Most studies to date have evaluated these structures qualitatively, whereas this paper aims to provide a quantitative method of examining changes in these structures by fluorescently labeling the element of interest and performing single cell image analysis. The method described herein tracks the diameter of the microtubule ring and the circumference of the actin matrix as they change over time. Platelets were incubated with a series of drugs that interact with tubulin or actin, and the platelets were observed for variation in shape change dynamics throughout the activation process. Differences in shape change mechanics due to drug incubation were observable in each case.

Item Type:Article
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URLURL TypeDescription Material CentralArticle
Additional Information:© 2015 Springer-Verlag Berlin Heidelberg. Received: 21 November 2014. Revised: 25 January 2015. Accepted: 27 January 2015. Published online: 21 February 2015.
Subject Keywords:Platelet Activation Fluorescence imaging Cytoskeleton
PubMed Central ID:PMC4370791
Record Number:CaltechAUTHORS:20170616-092006534
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Official Citation:Finkenstaedt-Quinn, S.A., Ge, S. & Haynes, C.L. Anal Bioanal Chem (2015) 407: 2803. doi:10.1007/s00216-015-8523-7
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:78262
Deposited By: Ruth Sustaita
Deposited On:16 Jun 2017 16:43
Last Modified:16 Jun 2017 16:43

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