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Uncharacterized bacterial structures revealed by electron cryotomography

Dobro, Megan J. and Oikonomou, Catherine M. and Piper, Aidan and Cohen, John and Guo, Kylie and Jensen, Taylor and Tadayon, Jahan and Donermeyer, Joseph and Park, Yeram and Solis, Benjamin A. and Kjær, Andreas and Jewett, Andrew I. and McDowall, Alasdair W. and Chen, Songye and Chang, Yi-Wei and Shi, Jian and Subramanian, Poorna and Iancu, Cristina V. and Li, Zhuo and Briegel, Ariane and Tocheva, Elitza I. and Pilhofer, Martin and Jensen, Grant J. (2017) Uncharacterized bacterial structures revealed by electron cryotomography. Journal of Bacteriology, 199 (17). Art. No. e00100-17. ISSN 0021-9193. PMCID PMC5553035. https://resolver.caltech.edu/CaltechAUTHORS:20170616-093425610

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Abstract

Electron cryotomography (ECT) can reveal the native structure and arrangement of macromolecular complexes inside intact cells. This technique has greatly advanced our understanding of the ultrastructure of bacterial cells. We now view bacteria as structurally complex assemblies of macromolecular machines rather than as undifferentiated bags of enzymes. To date, our group has applied ECT to nearly 90 different bacterial species, collecting more than 15,000 cryotomograms. In addition to known structures, we have observed, to our knowledge, several uncharacterized features in these tomograms. Some are completely novel structures; others expand the features or species range of known structure types. Here, we present a survey of these uncharacterized bacterial structures in the hopes of accelerating their identification and study, and furthering our understanding of the structural complexity of bacterial cells.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1128/JB.00100-17DOIArticle
http://jb.asm.org/content/199/17/e00100-17PublisherArticle
https://doi.org/10.1101/108191DOIDiscussion Paper
http://jb.asm.org/content/199/17/e00100-17/suppl/DCSupplementalPublisherSupplemental Material
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553035/PubMed CentralArticle
ORCID:
AuthorORCID
Dobro, Megan J.0000-0002-6464-3932
Oikonomou, Catherine M.0000-0003-2312-4746
Cohen, John0000-0003-1772-8236
Jensen, Taylor0000-0002-7503-9079
Tadayon, Jahan0000-0002-2075-6345
Park, Yeram0000-0002-6553-5722
Solis, Benjamin A.0000-0002-6428-2903
Kjær, Andreas0000-0002-0096-5764
Jewett, Andrew I.0000-0002-6252-9888
Chen, Songye0000-0001-5407-5049
Chang, Yi-Wei0000-0003-2391-473X
Shi, Jian0000-0003-3810-5302
Iancu, Cristina V.0000-0002-7352-9226
Briegel, Ariane0000-0003-3733-3725
Tocheva, Elitza I.0000-0002-4869-8319
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2017 American Society for Microbiology. Received 10 March 2017; Accepted 27 May 2017; Accepted manuscript posted online 12 June 2017. We thank our collaborators who provided strains for imaging: Andrew Camilli (Streptococcus pneumoniae), Eric Matson (strain JT5), Gladys Alexandre (Azospirillum brasilense mutants), Lotte Søgaard-Andersen, Simon Ringgaard, and Matthew K. Waldor (Vibrio cholerae wild type and mutants), Michael Marletta (Shewanella putrefaciens), and Gordon Cannon and Sabine Heinhorst (Halothiobacillus neapolitanus and Thiomonas intermedia). We also thank members of the Jensen lab for their helpful discussions. M.J.D. and G.J.J. conceived the idea for the study; M.J.D., C.M.O., A.P., J.C., K.G., T.J., J.T., J.D., Y.P., A.K., A.I.J., M.P., S.C., E.I.T., Y.-W.C., A.B., J.S., Z.L., P.S., C.V.I., B.A.S., and A.W.M. performed formal analysis and investigation; M.J.D. and C.M.O. wrote and prepared the original article draft; M.J.D., C.M.O., and G.J.J. wrote, reviewed, and edited the final article draft; M.J.D. and G.J.J. acquired funding; G.J.J. provided resources; and M.J.D. and G.J.J. supervised the study. This work was supported by the Hampshire College Dr. Lucy fund and the Collaborative Modeling Center, NIH grant R01 AI27401 (to G.J.J.), the Beckman Institute at Caltech, the Gordon and Betty Moore Foundation, the Human Frontier Science Program, the Howard Hughes Medical Institute, and the John Templeton Foundation as part of the Boundaries of Life project. The opinions expressed in this publication are those of the authors and do not necessarily reflect the views of the John Templeton Foundation. We declare no competing interests.
Funders:
Funding AgencyGrant Number
Hampshire CollegeUNSPECIFIED
NIHR01 AI27401
Caltech Beckman InstituteUNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Human Frontier Science ProgramUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
John Templeton FoundationUNSPECIFIED
Subject Keywords:bacteria, electron cryotomography, cryo-EM, bacterial ultrastructure, uncharacterized structures
Issue or Number:17
PubMed Central ID:PMC5553035
Record Number:CaltechAUTHORS:20170616-093425610
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170616-093425610
Official Citation:Dobro MJ, Oikonomou CM, Piper A, Cohen J, Guo K, Jensen T, Tadayon J, Donermeyer J, Park Y, Solis BA, Kjær A, Jewett AI, McDowall AW, Chen S, Chang Y-W, Shi J, Subramanian P, Iancu CV, Li Z, Briegel A, Tocheva EI, Pilhofer M, Jensen GJ. 2017. Uncharacterized bacterial structures revealed by electron cryotomography. J Bacteriol 199:e00100-17. https://doi.org/10.1128/JB.00100-17.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:78264
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Jun 2017 16:55
Last Modified:04 Oct 2019 22:56

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