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SUPERMAN prevents class B gene expression and promotes stem cell termination in the fourth whorl of Arabidopsis thaliana flowers

Prunet, Nathanaël and Yang, Weibing and Das, Pradeep and Meyerowitz, Elliot M. and Jack, Thomas P. (2017) SUPERMAN prevents class B gene expression and promotes stem cell termination in the fourth whorl of Arabidopsis thaliana flowers. Proceedings of the National Academy of Sciences of the United States of America, 114 (27). pp. 7166-7171. ISSN 0027-8424. PMCID PMC5502645. https://resolver.caltech.edu/CaltechAUTHORS:20170622-093332240

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Abstract

The molecular and genetic networks underlying the determination of floral organ identity are well studied, but much less is known about how the flower is partitioned into four developmentally distinct whorls. The SUPERMAN gene is required for proper specification of the boundary between stamens in whorl 3 and carpels in whorl 4, as superman mutants exhibit supernumerary stamens but usually lack carpels. However, it has remained unclear whether extra stamens in superman mutants originate from an organ identity change in whorl 4 or the overproliferation of whorl 3. Using live confocal imaging, we show that the extra stamens in superman mutants arise from cells in whorl 4, which change their fate from female to male, while floral stem cells proliferate longer, allowing for the production of additional stamens.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.1705977114DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5502645/PubMed CentralArticle
http://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1705977114/-/DCSupplementalPublisherSupporting Information
https://doi.org/10.1101/107722DOIDiscussion Paper
ORCID:
AuthorORCID
Prunet, Nathanaël0000-0002-8939-5920
Yang, Weibing0000-0002-2379-5729
Meyerowitz, Elliot M.0000-0003-4798-5153
Alternate Title:SUPERMAN prevents stamen formation and promotes stem cell termination in the fourth whorl of the Arabidopsis flower
Additional Information:© 2017 National Academy of Sciences. Contributed by Elliot M. Meyerowitz, May 19, 2017 (sent for review April 11, 2017; reviewed by Richard G. H. Immink and David Smyth). Published online before print June 20, 2017. We thank John Chandler and Paul Tarr for providing reporter lines, Toshiro Ito and Frank Wellmer for critical reading of the manuscript, and Ann Lavanway for her tremendous help with live confocal imaging. Funding in the T.P.J. Laboratory was provided by National Science Foundation Grant IOS-0926347; and funding in the E.M.M. Laboratory was provided by the Howard Hughes Medical Institute, the National Institutes of Health Grant R01 GM104244, and the Gordon and Betty Moore Foundation through Grant GBMF3406. Author contributions: N.P., P.D., E.M.M., and T.P.J. designed the experiments; N.P., W.Y., and T.P.J. performed the experiments; N.P. and T.P.J. generated the reporter lines; N.P. performed the live confocal imaging; W.Y. performed the in situ hybridizations; N.P. wrote the paper; and P.D., E.M.M., and T.P.J. edited versions of the paper. Reviewers: R.G.H.I., Wageningen Plant Research; and D.S., Monash University. The authors declare no conflict of interest. This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1705977114/-/DCSupplemental.
Funders:
Funding AgencyGrant Number
NSFIOS-0926347
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
NIHR01 GM104244
Gordon and Betty Moore FoundationGBMF3406
Subject Keywords:flower development; boundary formation; floral organ identity; stem cells; SUPERMAN
Issue or Number:27
PubMed Central ID:PMC5502645
Record Number:CaltechAUTHORS:20170622-093332240
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170622-093332240
Official Citation:Nathanaël Prunet, Weibing Yang, Pradeep Das, Elliot M. Meyerowitz, and Thomas P. Jack SUPERMAN prevents class B gene expression and promotes stem cell termination in the fourth whorl of Arabidopsis thaliana flowers PNAS 2017 114 (27) 7166-7171; published ahead of print June 20, 2017, doi:10.1073/pnas.1705977114
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:78461
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:22 Jun 2017 17:39
Last Modified:09 Mar 2020 13:19

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