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Light-Dependent Regulation of Sleep and Wake States by Prokineticin 2 in Zebrafish

Chen, Shijia and Reichert, Sabine and Singh, Chanpreet and Oikonomou, Grigorios and Rihel, Jason and Prober, David A. (2017) Light-Dependent Regulation of Sleep and Wake States by Prokineticin 2 in Zebrafish. Neuron, 95 (1). pp. 153-168. ISSN 0896-6273. PMCID PMC5653285. doi:10.1016/j.neuron.2017.06.001.

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Light affects sleep and wake behaviors by providing an indirect cue that entrains circadian rhythms and also by inducing a direct and rapid regulation of behavior. While circadian entrainment by light is well characterized at the molecular level, mechanisms that underlie the direct effect of light on behavior are largely unknown. In zebrafish, a diurnal vertebrate, we found that both overexpression and mutation of the neuropeptide prokineticin 2 (Prok2) affect sleep and wake behaviors in a light-dependent but circadian-independent manner. In light, Prok2 overexpression increases sleep and induces expression of galanin (galn), a hypothalamic sleep-inducing peptide. We also found that light-dependent, Prok2-induced sedation requires prokineticin receptor 2 (prokr2) and is strongly suppressed in galn mutants. These results suggest that Prok2 antagonizes the direct wake-promoting effect of light in zebrafish, in part through the induction of galn expression in the hypothalamus.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Singh, Chanpreet0000-0002-2697-3033
Oikonomou, Grigorios0000-0001-6797-7375
Rihel, Jason0000-0003-4067-2066
Prober, David A.0000-0002-7371-4675
Alternate Title:Light-dependent regulation of sleep/wake states by prokineticin 2 in zebrafish
Additional Information:© 2017 Elsevier B.V. Received 25 January 2017, Revised 24 April 2017, Accepted 31 May 2017, Available online 22 June 2017. We thank Daisy Chilin, Alex Mack Cruz, and Kenna Molinder for animal husbandry, Viveca Sapin, Brett Niles, and Jae Chu for genotyping and Catherine Oikonomou and members of the Prober lab for comments on the manuscript. This work was supported by grants from the NIH (S.C.: NS077842; D.A.P.: NS070911, NS094390, and NS095824), the Mallinckrodt, Rita Allen and Brain and Behavior Research Foundations (D.A.P.), a Sir Henry Wellcome Trust Fellowship (S.R.), a UCL Excellence Fellowship (J.R.), and a European Research Council Starting Grant (J.R.: 282027).
Group:Tianqiao and Chrissy Chen Institute for Neuroscience
Funding AgencyGrant Number
Mallinckrodt FoundationUNSPECIFIED
Rita Allen FoundationUNSPECIFIED
Brain and Behavior Research FoundationUNSPECIFIED
University College LondonUNSPECIFIED
European Research Council (ERC)282027
Subject Keywords:prokineticin 2; galanin; sleep; circadian rhythm; masking; zebrafish
Issue or Number:1
PubMed Central ID:PMC5653285
Record Number:CaltechAUTHORS:20170623-125222628
Persistent URL:
Official Citation:Shijia Chen, Sabine Reichert, Chanpreet Singh, Grigorios Oikonomou, Jason Rihel, David A. Prober, Light-Dependent Regulation of Sleep and Wake States by Prokineticin 2 in Zebrafish, Neuron, Volume 95, Issue 1, 5 July 2017, Pages 153-168.e6, ISSN 0896-6273,
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:78520
Deposited By: Tony Diaz
Deposited On:23 Jun 2017 22:18
Last Modified:25 Mar 2022 17:59

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