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High-resolution deep functional imaging of the whole mouse brain by photoacoustic computed tomography in vivo

Zhang, Pengfei and Li, Lei and Lin, Li and Hu, Peng and Shi, Junhui and He, Yun and Zhu, Liren and Zhou, Yong and Wang, Lihong V. (2018) High-resolution deep functional imaging of the whole mouse brain by photoacoustic computed tomography in vivo. Journal of Biophotonics, 11 (1). Art. No. e201700024. ISSN 1864-063X. PMCID PMC5777675. doi:10.1002/jbio.201700024.

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Photoacoustic computed tomography (PACT) is a non-invasive imaging technique offering high contrast, high resolution, and deep penetration in biological tissues. We report a PACT system equipped with a high frequency linear transducer array for mapping the microvascular network of a whole mouse brain with the skull intact and studying its hemodynamic activities. The linear array was scanned in the coronal plane to collect data from different angles, and full-view images were synthesized from the limited-view images in which vessels were only partially revealed. We investigated spontaneous neural activities in the deep brain by monitoring the concentration of hemoglobin in the blood vessels and observed strong interhemispherical correlations between several chosen functional regions, both in the cortical layer and in the deep regions. We also studied neural activities during an epileptic seizure and observed the epileptic wave spreading around the injection site and the wave propagating in the opposite hemisphere.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Li, Lei0000-0001-6164-2646
Lin, Li0000-0002-0517-8436
Hu, Peng0000-0002-2933-1239
Shi, Junhui0000-0002-5741-2781
He, Yun0000-0001-8430-6370
Zhu, Liren0000-0002-6338-9338
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Version of record online: 21 June 2017; Manuscript Accepted: 3 May 2017; Manuscript Revised: 2 May 2017; Manuscript Received: 29 January 2017. This work was funded by the National Institutes of Health (NIH) Grants U01 NS090579 (NIH Brain Initiative Award), U01 NS099717 (NIH Brain Initiative Award), R01 EB016963, DP1 EB016986 (Director's Pioneer Award), R01 CA186567 (Director's Transformative Research Award), S10 RR026922. The authors thank Prof. James Ballard for his close reading of the manuscript. L. V. Wang has a financial interest in Microphotoacoustics, Inc., which, however, did not support this work.
Funding AgencyGrant Number
NIHU01 NS090579
NIHU01 NS099717
NIHR01 EB016963
NIHDP1 EB016986
NIHR01 CA186567
NIHS10 RR026922
Subject Keywords:Photoacoustic computed tomography; deep mouse brain; functional imaging
Issue or Number:1
PubMed Central ID:PMC5777675
Record Number:CaltechAUTHORS:20170627-080108636
Persistent URL:
Official Citation:Zhang, P., Li, L., Lin, L., Hu, P., Shi, J., He, Y., Zhu, L., Zhou, Y. and Wang, L. V. (2018), High-resolution deep functional imaging of the whole mouse brain by photoacoustic computed tomography in vivo. J. Biophotonics, 11: n/a, e201700024. doi:10.1002/jbio.201700024
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:78589
Deposited By: Tony Diaz
Deposited On:27 Jun 2017 20:17
Last Modified:15 Nov 2021 17:41

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