CaltechAUTHORS
  A Caltech Library Service

Engineering pulsatile communication in bacterial consortia

Parkin, James M. and Hsiao, Victoria and Murray, Richard M. (2017) Engineering pulsatile communication in bacterial consortia. . (Submitted) http://resolver.caltech.edu/CaltechAUTHORS:20170627-105933916

[img] PDF - Submitted Version
Creative Commons Attribution.

2609Kb
[img] Archive (ZIP) (Mathematica model notebook) - Supplemental Material
Creative Commons Attribution.

6Mb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20170627-105933916

Abstract

Lux-type quorum sensing systems enable communication in bacteria with only two protein components: a signal synthase and an inducible transcription activator. The simplicity of these systems makes them a popular choice for engineering collaborative behaviors in synthetic bacterial consortia, such as photographic edge detection and synchronized oscillation. To add to this body of work, we propose a pulsatile communication circuit that enables dynamic patterning and long-distance communication analogous to action potentials traveling through nerve tissue. We employed a model-driven design paradigm involving incremental characterization of in vivo design candidates with increasing circuit complexity. Beginning with a simple inducible reporter system, we screened a small number of circuits varying in their promoter and ribosomal binding site strengths. From this candidate pool, we selected a candidate to be the seed network for the subsequent round of more complex circuit variants, likewise variable in promoter and RBS strengths. The selection criteria at each level of complexity is tailored to optimize a different desirable performance characteristic. By this approach we individually optimized reporter signal-to-background ratio, pulsatile response to induction, and quiescent basal transcription, avoiding large library screens while ensuring robust performance of the composite circuit.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/111906 DOIDiscussion Paper
http://biorxiv.org/content/early/2017/02/26/111906OrganizationDiscussion Paper
ORCID:
AuthorORCID
Hsiao, Victoria0000-0001-9297-1522
Murray, Richard M.0000-0002-5785-7481
Additional Information:The copyright holder for this preprint is the author/funder. It is made available under a CC-BY-ND 4.0 International license. bioRxiv preprint first posted online Feb. 26, 2017. James M. Parkin is supported by the Institute for Collaborative Biotechnologies through grant W911NF-09-0001 from the U.S. Army Research Office. The content of the information does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred. Plasmid vectors and non-coding regions were provided as a generous gift of Douglas Densmore at the Cross-disciplinary Integration of Design Automation Research lab (Addgene Kit # 1000000059 ). Quorum sensing promoters and coding sequences were provided as a generous gift from Matthew Bennet (Addgene Plasmid # 65954, 65952).
Funders:
Funding AgencyGrant Number
Army Research Office (ARO)W911NF-09-0001
Record Number:CaltechAUTHORS:20170627-105933916
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20170627-105933916
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:78611
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:27 Jun 2017 19:30
Last Modified:27 Jun 2017 19:30

Repository Staff Only: item control page