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Theory of long binding events in single-molecule–controlled rotation experiments on F_1-ATPase

Volkán-Kacsó, Sándor and Marcus, Rudolph A. (2017) Theory of long binding events in single-molecule–controlled rotation experiments on F_1-ATPase. Proceedings of the National Academy of Sciences of the United States of America, 114 (28). pp. 7272-7277. ISSN 0027-8424. https://resolver.caltech.edu/CaltechAUTHORS:20170628-102612597

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Abstract

The theory of elastic group transfer for the binding and release rate constants for nucleotides in F_1-ATPase as a function of the rotor angle is further extended in several respects. (i) A method is described for predicting the experimentally observed lifetime distribution of long binding events in the controlled rotation experiments by taking into account the hydrolysis and synthesis reactions occurring during these events. (ii) A method is also given for treating the long binding events in the experiments and obtaining the rate constants for the hydrolysis and synthesis reactions occurring during these events. (iii) The theory in the previous paper is given in a symmetric form, an extension that simplifies the application of the theory to experiments. It also includes a theory-based correction of the reported “on” and “off” rates by calculating the missed events. A near symmetry of the data about the angle of −40° and a “turnover” in the binding rate data vs. rotor angle for angles greater than ∼∼40° is also discussed.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.1705960114DOIArticle
http://www.pnas.org/content/114/28/7272PublisherArticle
http://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1705960114/-/DCSupplementalPublisherSupporting Information
ORCID:
AuthorORCID
Marcus, Rudolph A.0000-0001-6547-1469
Additional Information:© 2017 National Academy of Sciences. Contributed by Rudolph A. Marcus, May 17, 2017 (sent for review April 10, 2017; reviewed by Attila Szabo and Arieh Warshel). Published online before print June 26, 2017. We thank Drs. Hiroyuki Noji and Kengo Adachi for helpful discussions and comments. This work was supported by the Office of the Naval Research, the Army Research Office, and the James W. Glanville Foundation. Author contributions: S.V.-K. and R.A.M. designed research; S.V.-K. and R.A.M. performed research; S.V.-K. contributed new reagents/analytic tools; S.V.-K. and R.A.M. analyzed data; and S.V.-K. and R.A.M. wrote the paper. The authors declare no conflict of interest. This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1705960114/-/DCSupplemental.
Funders:
Funding AgencyGrant Number
Office of Naval Research (ONR)UNSPECIFIED
Army Research Office (ARO)UNSPECIFIED
James W. Glanville FoundationUNSPECIFIED
Subject Keywords:F-ATPase; single-molecule imaging; nucleotide binding; hydrolysis; group transfer theory
Issue or Number:28
Record Number:CaltechAUTHORS:20170628-102612597
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170628-102612597
Official Citation:Sándor Volkán-Kacsó and Rudolph A. Marcus Theory of long binding events in single-molecule–controlled rotation experiments on F1-ATPase PNAS 2017 114 (28) 7272-7277; published ahead of print June 26, 2017, doi:10.1073/pnas.1705960114
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:78642
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:28 Jun 2017 18:19
Last Modified:22 Nov 2019 09:58

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