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Phospholipase Cβ4 is specifically involved in climbing fiber synapse elimination in the developing cerebellum

Kano, Masanobu and Hashimoto, Kouichi and Watanabe, Masahiko and Kurihara, Hideo and Offermanns, Stefan and Jiang, Huiping and Wu, Yanping and Jun, Kisun and Shin, Hee-Sup and Inoue, Yoshiro and Simon, Melvin I. and Wu, Dianqing (1998) Phospholipase Cβ4 is specifically involved in climbing fiber synapse elimination in the developing cerebellum. Proceedings of the National Academy of Sciences of the United States of America, 95 (26). pp. 15724-15729. ISSN 0027-8424. PMCID PMC28111.

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Elimination of excess climbing fiber (CF)-Purkinje cell synapses during cerebellar development involves a signaling pathway that includes type 1 metabotropic glutamate receptor, Galpha q, and the gamma isoform of protein kinase C. To identify phospholipase C (PLC) isoforms involved in this process, we generated mice deficient in PLCbeta 4, one of two major isoforms expressed in Purkinje cells. PLCbeta 4 mutant mice are viable but exhibit locomotor ataxia. Their cerebellar histology, parallel fiber synapse formation, and basic electrophysiology appear normal. However, developmental elimination of multiple CF innervation clearly is impaired in the rostral portion of the cerebellar vermis, in which PLCbeta 4 mRNA is predominantly expressed. By contrast, CF synapse elimination is normal in the caudal cerebellum, in which low levels of PLCbeta 4 mRNA but reciprocally high levels of PLCbeta 3 mRNA are found. These results indicate that PLCbeta 4 transduces signals that are required for CF synapse elimination in the rostral cerebellum

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Additional Information:© 1998 by The National Academy of Sciences Contributed by Melvin I. Simon, October 12, 1998 We thank K. Matsumoto and V. Mancino for expert technical help and Dr. N. Kawai for continuous encouragement. This work has been supported by grants to M.K. from the Japanese Ministry of Education, Science, Sports and Culture, from the Human Frontier Science Program, and by Special Coordination Funds for promoting Science and Technology from the Science and Technology Agency of the Japanese Government. This work also has been supported by a grant to H.-S.S. from the Creative Research Initiatives of the Korean Government and by grants to D.W. and to M.I.S. from the National Institutes of Health. S.O. was a recipient of a fellowship from the Deutsche Forschungsgemeinschaft and the Guenther Foundation. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
Ministry of Education, Culture, Sports, Science and Technology (MEXT)UNSPECIFIED
Human Frontier Science ProgramUNSPECIFIED
Japan Science and Technology AgencyUNSPECIFIED
National Research Foundation of KoreaUNSPECIFIED
Deutsche Forschungsgemeinschaft (DFG)UNSPECIFIED
Guenther FoundationUNSPECIFIED
Issue or Number:26
PubMed Central ID:PMC28111
Record Number:CaltechAUTHORS:KANpnas98
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:787
Deposited By: Archive Administrator
Deposited On:30 Sep 2005
Last Modified:02 Oct 2019 22:36

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