CaltechAUTHORS
  A Caltech Library Service

Electrical Probes of DNA-Binding Proteins

Barton, Jacqueline K. and Bartels, Phillip L. and Deng, Yingxin and O'Brien, Elizabeth (2017) Electrical Probes of DNA-Binding Proteins. In: DNA Repair Enzymes: Cell, Molecular, and Chemical Biology. Methods in Enzymology. Vol.591. No.591. Academic Press , San Diego, CA, pp. 355-414. ISBN 9780128118467. https://resolver.caltech.edu/CaltechAUTHORS:20170705-093949953

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20170705-093949953

Abstract

A DNA electrochemistry platform has been developed to probe proteins bound to DNA electrically. Here gold electrodes are modified with thiol-modified DNA, and DNA charge transport chemistry is used to probe DNA binding and enzymatic reaction both with redox-silent and redox-active proteins. For redox-active proteins, the electrochemistry permits the determination of redox potentials in the DNA-bound form, where comparisons to DNA-free potentials can be made using graphite electrodes without DNA modification. Importantly, electrochemistry on the DNA-modified electrodes facilitates reaction under aqueous, physiological conditions with a sensitive electrical measurement of binding and activity.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/bs.mie.2017.03.024DOIArticle
http://www.sciencedirect.com/science/article/pii/S0076687917301222PublisherArticle
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© Academic Press 2017. Available online 10 May 2017. Published: 20th June 2017. We are grateful to the NIH for their support of this research and to our coworkers and collaborators for their insights, efforts, and hard work.
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
Subject Keywords:DNA glycosylase; Nucleotide flipping; Base flipping; Transient kinetics; DNA binding; DNA modification; Kinetic simulation; Stopped flow; DNA alkylation repair; Base excision repair
Series Name:Methods in Enzymology
Issue or Number:591
Record Number:CaltechAUTHORS:20170705-093949953
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170705-093949953
Official Citation:Jacqueline K. Barton, Phillip L. Bartels, Yingxin Deng, Elizabeth O'Brien, Chapter Fifteen - Electrical Probes of DNA-Binding Proteins, In: Brandt F. Eichman, Editor(s), Methods in Enzymology, Academic Press, 2017, Volume 591, Pages 355-414, ISSN 0076-6879, ISBN 9780128118467, https://doi.org/10.1016/bs.mie.2017.03.024. (http://www.sciencedirect.com/science/article/pii/S0076687917301222)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:78756
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:06 Jul 2017 21:42
Last Modified:03 Oct 2019 18:11

Repository Staff Only: item control page