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Electrical Probes of DNA-Binding Proteins

Barton, Jacqueline K. and Bartels, Phillip L. and Deng, Yingxin and O'Brien, Elizabeth (2017) Electrical Probes of DNA-Binding Proteins. In: DNA Repair Enzymes: Cell, Molecular, and Chemical Biology. Methods in Enzymology. Vol.591. No.591. Academic Press , San Diego, CA, pp. 355-414. ISBN 9780128118467. PMCID PMC6314295.

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A DNA electrochemistry platform has been developed to probe proteins bound to DNA electrically. Here gold electrodes are modified with thiol-modified DNA, and DNA charge transport chemistry is used to probe DNA binding and enzymatic reaction both with redox-silent and redox-active proteins. For redox-active proteins, the electrochemistry permits the determination of redox potentials in the DNA-bound form, where comparisons to DNA-free potentials can be made using graphite electrodes without DNA modification. Importantly, electrochemistry on the DNA-modified electrodes facilitates reaction under aqueous, physiological conditions with a sensitive electrical measurement of binding and activity.

Item Type:Book Section
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URLURL TypeDescription CentralArticle
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© Academic Press 2017. Available online 10 May 2017. Published: 20th June 2017. We are grateful to the NIH for their support of this research and to our coworkers and collaborators for their insights, efforts, and hard work.
Funding AgencyGrant Number
Subject Keywords:DNA glycosylase; Nucleotide flipping; Base flipping; Transient kinetics; DNA binding; DNA modification; Kinetic simulation; Stopped flow; DNA alkylation repair; Base excision repair
Series Name:Methods in Enzymology
Issue or Number:591
PubMed Central ID:PMC6314295
Record Number:CaltechAUTHORS:20170705-093949953
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Official Citation:Jacqueline K. Barton, Phillip L. Bartels, Yingxin Deng, Elizabeth O'Brien, Chapter Fifteen - Electrical Probes of DNA-Binding Proteins, In: Brandt F. Eichman, Editor(s), Methods in Enzymology, Academic Press, 2017, Volume 591, Pages 355-414, ISSN 0076-6879, ISBN 9780128118467, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:78756
Deposited By: Ruth Sustaita
Deposited On:06 Jul 2017 21:42
Last Modified:15 Nov 2021 17:42

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