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Advanced electron paramagnetic resonance on the catalytic iron–sulfur cluster bound to the CCG domain of heterodisulfide reductase and succinate: quinone reductase

Fielding, Alistair J. and Parey, Kristian and Ermler, Ulrich and Scheller, Silvan and Jaun, Bernhard and Bennati, Marina (2013) Advanced electron paramagnetic resonance on the catalytic iron–sulfur cluster bound to the CCG domain of heterodisulfide reductase and succinate: quinone reductase. Journal of Biological Inorganic Chemistry, 18 (8). pp. 905-915. ISSN 0949-8257. https://resolver.caltech.edu/CaltechAUTHORS:20170705-102745214

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Abstract

Heterodisulfide reductase (Hdr) is a key enzyme in the energy metabolism of methanogenic archaea. The enzyme catalyzes the reversible reduction of the heterodisulfide (CoM-S-S-CoB) to the thiol coenzymes M (CoM-SH) and B (CoB-SH). Cleavage of CoM-S-S-CoB at an unusual FeS cluster reveals unique substrate chemistry. The cluster is fixed by cysteines of two cysteine-rich CCG domain sequence motifs (CX_(31–39)CCX_(35–36)CXXC) of subunit HdrB of the Methanothermobacter marburgensis HdrABC complex. We report on Q-band (34 GHz) ^(57)Fe electron-nuclear double resonance (ENDOR) spectroscopic measurements on the oxidized form of the cluster found in HdrABC and in two other CCG-domain-containing proteins, recombinant HdrB of Hdr from M. marburgensis and recombinant SdhE of succinate: quinone reductase from Sulfolobus solfataricus P2. The spectra at 34 GHz show clearly improved resolution arising from the absence of proton resonances and polarization effects. Systematic spectral simulations of 34 GHz data combined with previous 9 GHz data allowed the unambiguous assignment of four ^(57)Fe hyperfine couplings to the cluster in all three proteins. ^(13)C Mims ENDOR spectra of labelled CoM-SH were consistent with the attachment of the substrate to the cluster in HdrABC, whereas in the other two proteins no substrate is present. ^(57)Fe resonances in all three systems revealed unusually large ^(57)Fe ENDOR hyperfine splitting as compared to known systems. The results infer that the cluster’s unique magnetic properties arise from the CCG binding motif.


Item Type:Article
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http://dx.doi.org/10.1007/s00775-013-1037-xDOIArticle
https://link.springer.com/article/10.1007/s00775-013-1037-xPublisherArticle
http://rdcu.be/tZcUPublisherFree ReadCube access
ORCID:
AuthorORCID
Scheller, Silvan0000-0002-0667-9224
Additional Information:© SBIC 2013. Received: 31 May 2013. Accepted: 21 August 2013. Published online: 14 September 2013. This work was supported by the Deutsche Forschungsgemeinschaft (DFG) priority program SPP 1319 and the Max Planck Society. We thank R. Hedderich and N. Hamann for providing the constructs of HdrB and SdhE and Prof. R. Thauer for discussion and financial support of K. Parey.
Funders:
Funding AgencyGrant Number
Deutsche Forschungsgemeinschaft (DFG)SPP 1319
Max Planck SocietyUNSPECIFIED
Subject Keywords:ENDOR; Iron–sulfur cluster; Heterodisulfide reductase; EPR
Issue or Number:8
Record Number:CaltechAUTHORS:20170705-102745214
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170705-102745214
Official Citation:Fielding, A.J., Parey, K., Ermler, U. et al. J Biol Inorg Chem (2013) 18: 905. doi:10.1007/s00775-013-1037-x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:78760
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:06 Jul 2017 22:58
Last Modified:03 Oct 2019 23:45

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