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LIF Is an Autocrine Factor for Sympathetic Neurons

Cheng, Jr-Gang and Patterson, Paul H. (1997) LIF Is an Autocrine Factor for Sympathetic Neurons. Molecular and Cellular Neuroscience, 9 (5-6). pp. 372-380. ISSN 1044-7431. doi:10.1006/mcne.1997.0635.

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Leukemia inhibitory factor (LIF) alters neuronal phenotypes bothin vitroandin vivo.Since it can be produced by glia and other nonneural cells, LIF is a candidate target-derived differentiation factor as well as an injury-response factor. We here provide evidence that LIF can be produced by neurons and can act on the neurons that produce it. A reverse transcriptase–polymerase chain reaction assay detects LIF mRNA in rat sympathetic neuron cultures, andin situhybridization combined with MAP2 immunocytochemistry indicates that most of the cells expressing LIF mRNA are, in fact, neurons. The neuronal lysate as well as the conditioned medium contains proteins that are specifically recognized by anti-LIF antibodies, and these antibodies also specifically stain the cultured neurons. In addition, concentrated sympathetic neuron conditioned medium can mimic the effects of LIF, and incubation of high-density sympathetic neuron cultures with anti-LIF antibodies reduces basal expression levels of LIF target genes such as particular neuropeptides, indicating that the endogenously produced cytokine is acting on the neurons under these conditions. Since we show that LIF transcript is expressed in sympathetic and sensory neuronsin vivoas well, LIF could act in an autocrine fashion under a variety of physiological conditions.

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Additional Information:© 1997 Academic Press. Received 25 April 1997, Revised 19 August 1997, Accepted 19 August 1997. We thank Reto Gadient and Rainer Lemke for assistance with in situ hybridization. This work was supported by a grant from NINDS to PHP.
Funding AgencyGrant Number
National Institute of Neurological Disorders and Stroke (NINDS)UNSPECIFIED
Subject Keywords:gp130; CNTF; leukemia inhibitory factor
Issue or Number:5-6
Record Number:CaltechAUTHORS:20170707-144354635
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Official Citation:Jr-Gang Cheng, Paul H. Patterson, LIF Is an Autocrine Factor for Sympathetic Neurons, Molecular and Cellular Neuroscience, Volume 9, Issue 5, 1997, Pages 372-380, ISSN 1044-7431, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:78869
Deposited By: Tony Diaz
Deposited On:10 Jul 2017 17:42
Last Modified:15 Nov 2021 17:43

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