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Gain‐ and Loss‐of‐Function Approaches in the Chick Embryo

Sauka‐Spengler, Tatjana and Barembaum, Meyer (2008) Gain‐ and Loss‐of‐Function Approaches in the Chick Embryo. In: Avian Embryology, 2nd Edition. Methods in Cell Biology . No.87. Elsevier , pp. 237-256. ISBN 978-0-12-564174-6.

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The chicken embryo has been used as a classical embryological model for studying developmental events because of its ready availability, similarity to the human embryos, and amenability to embryological and surgical manipulations. With the arrival of the molecular era, however, avian embryos presented distinct experimental limitations, largely because of the difficulty of performing targeted mutagenesis or transgenic studies. However, in the last decade and a half, a number of new methods for transient transgenesis have been developed that allow efficient alteration of gene function during early embryonic development. These techniques have made it possible to study the effects of gene inactivation or overexpression on downstream transcriptional regulation as well as on embryonic derivatives. This, together with sequencing of the chicken genome, has allowed the chicken embryo to enter the genomic era. While attempts to establish germ line transgenesis are ongoing, methods for rapid, transient spatiotemporally targeted gene alterations have thus again re‐established the chick embryo as an important experimental niche by making it possible to apply genetics in concert with classical embryological techniques. This provides a unique tool to explore the role of developmentally important genes (Ishii and Mikawa, 2005; Itasaki et al., 1999; Krull, 2004; Ogura, 2002; Swartz et al., 2001).

Item Type:Book Section
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Sauka‐Spengler, Tatjana0000-0001-9289-0263
Additional Information:© 2008 Elsevier Inc. Available online 14 May 2008.
Series Name:Methods in Cell Biology
Issue or Number:87
Record Number:CaltechAUTHORS:20170710-111549655
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Official Citation:Tatjana Sauka‐Spengler, Meyer Barembaum, Chapter 12 Gain‐ and Loss‐of‐Function Approaches in the Chick Embryo, Methods in Cell Biology, Academic Press, 2008, Volume 87, Pages 237-256, ISSN 0091-679X, ISBN 9780125641746, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:78893
Deposited By: Ruth Sustaita
Deposited On:10 Jul 2017 19:48
Last Modified:15 Nov 2021 17:43

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