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Characterizing Single Polymeric and Protein Nanoparticles with Surface Plasmon Resonance Imaging Measurements

Maley, Adam M. and Lu, George J. and Shapiro, Mikhail G. and Corn, Robert M. (2017) Characterizing Single Polymeric and Protein Nanoparticles with Surface Plasmon Resonance Imaging Measurements. ACS Nano, 11 (7). pp. 7447-7456. ISSN 1936-0851. PMCID PMC5531002.

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Near-infrared surface plasmon resonance imaging (SPRI) microscopy is used to detect and characterize the adsorption of single polymeric and protein nanoparticles (PPNPs) onto chemically modified gold thin films in real time. The single-nanoparticle SPRI responses, Δ%R_(NP), from several hundred adsorbed nanoparticles are collected in a single SPRI adsorption measurement. Analysis of Δ%R_(NP) frequency distribution histograms is used to provide information on the size, material content, and interparticle interactions of the PPNPs. Examples include the measurement of log-normal Δ%R_(NP) distributions for mixtures of polystyrene nanoparticles, the quantitation of bioaffinity uptake into and aggregation of porous NIPAm-based (N-isopropylacrylamide) hydrogel nanoparticles specifically engineered to bind peptides and proteins, and the characterization of the negative single-nanoparticle SPRI response and log-normal Δ%R_(NP) distributions obtained for three different types of genetically encoded gas-filled protein nanostructures derived from bacteria.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Maley, Adam M.0000-0003-1851-984X
Lu, George J.0000-0002-4689-9686
Shapiro, Mikhail G.0000-0002-0291-4215
Corn, Robert M.0000-0002-4756-2161
Additional Information:© 2017 American Chemical Society. Received: June 1, 2017; Accepted: July 10, 2017; Published: July 10, 2017. This work was supported by the National Science Foundation through grant CHE-1403506 (R.M.C.), the National Institutes of Health through grants R01-GM059622 (R.M.C.) and R01-EB018975 (M.G.S.), and the Heritage Medical Research Institute (M.G.S.). DLS data were acquired at the Laser Spectroscopy Facility in the Department of Chemistry at UCI. The authors acknowledge Y. Terada for the synthesis of p-acrylamidophenyl-α-d-mannopyranoside. The authors declare no competing financial interests.
Errata:The version of this paper that was published ASAP July 12, 2017, contained an error in eq 1. The corrected version was reposted July 13, 2017.
Group:Heritage Medical Research Institute
Funding AgencyGrant Number
Heritage Medical Research InstituteUNSPECIFIED
Subject Keywords:surface plasmon polaritons, single-nanoparticle refractive index, NIPAm-based hydrogel nanoparticle, melittin, Concanavalin A, gas vesicle, protein nanostructure
Issue or Number:7
PubMed Central ID:PMC5531002
Record Number:CaltechAUTHORS:20170711-140321159
Persistent URL:
Official Citation:Characterizing Single Polymeric and Protein Nanoparticles with Surface Plasmon Resonance Imaging Measurements Adam M. Maley, George J. Lu, Mikhail G. Shapiro, and Robert M. Corn ACS Nano 2017 11 (7), 7447-7456 DOI: 10.1021/acsnano.7b03859
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:78960
Deposited By: Tony Diaz
Deposited On:11 Jul 2017 21:10
Last Modified:22 Nov 2019 21:04

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