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Intraspecific variability in Phaeocystis antarctica's response to iron and light stress

Luxem, Katja E. and Ellwood, Michael J. and Strzepek, Robert F. (2017) Intraspecific variability in Phaeocystis antarctica's response to iron and light stress. PLoS ONE, 12 (7). Art. No. e0179751. ISSN 1932-6203. PMCID PMC5503234. https://resolver.caltech.edu/CaltechAUTHORS:20170717-092144983

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[img] MS Word (S3 Table. Intracellular chlorophyll a concentrations of the four P. antarctica clones grown under different iron- and light-conditions) - Supplemental Material
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[img] MS Word (S5 Table. Criteria used to determine whether clones exhibit a particular response to the iron limiting treatments) - Supplemental Material
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Abstract

Phaeocystis antarctica is an abundant phytoplankton species in the Southern Ocean, where growth is frequently limited by iron and light. Being able to grow under low iron conditions is essential to the species’ success, but there have been hints that this ability differs among clones. Here, we compare the growth, cell size and chlorophyll a concentrations of four P. antarctica clones cultured under different iron and light conditions. Iron was provided either as unchelated iron (Fe′) or bound to the bacterial siderophore desferrioxamine B, representing, respectively, the most and least bioavailable forms of iron which phytoplankton encounter in the marine environment. The growth rate data demonstrate that the clones vary in their ability to grow using organically bound iron, and that this ability is not related to their ability to grow at low inorganic iron concentrations. These results are consistent at low and high light. Physiologically, only three of the four clones shrink or decrease the concentration of chlorophyll a in response to iron limitation, and only one clone decreases colony formation. Together, our data show that P. antarctica clones 1) respond to the same degree of iron limitation using different acclimation strategies, and 2) vary in their ability to grow under the same external iron and light conditions. This physiological diversity is surprisingly large for isolates of a single phytoplankton species.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1371/journal.pone.0179751DOIArticle
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0179751PublisherArticle
https://doi.org/10.1371/journal.pone.0179751.s001DOIS1 Table
https://doi.org/10.1371/journal.pone.0179751.s002DOIS2 Table
https://doi.org/10.1371/journal.pone.0179751.s003DOIS3 Table
https://doi.org/10.1371/journal.pone.0179751.s004DOIS4 Table
https://doi.org/10.1371/journal.pone.0179751.s005DOIS5 Table
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5503234/PubMed CentralArticle
Additional Information:© 2017 Luxem et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received: November 27, 2016; Accepted: June 2, 2017; Published: July 10, 2017. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. KEL was supported by the Robert and Delpha Noland Summer Internship, which funded her travel to and accommodation in Australia. The Australian Research Council (DP130100679 to MJE) is acknowledged for funds to support this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors have declared that no competing interests exist. Author Contributions: Conceptualization: Katja E. Luxem, Michael J. Ellwood, Robert F. Strzepek. Formal analysis: Katja E. Luxem. Investigation: Katja E. Luxem, Robert F. Strzepek. Methodology: Katja E. Luxem, Michael J. Ellwood, Robert F. Strzepek. Visualization: Katja E. Luxem. Writing ± original draft: Katja E. Luxem. Writing ± review & editing: Katja E. Luxem, Michael J. Ellwood, Robert F. Strzepek.
Funders:
Funding AgencyGrant Number
Robert and Delpha Noland Summer InternshipUNSPECIFIED
Australian Research CouncilDP130100679
Issue or Number:7
PubMed Central ID:PMC5503234
Record Number:CaltechAUTHORS:20170717-092144983
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170717-092144983
Official Citation:Luxem KE, Ellwood MJ, Strzepek RF (2017) Intraspecific variability in Phaeocystis antarctica's response to iron and light stress. PLoS ONE 12(7): e0179751. https://doi.org/10.1371/journal.pone.0179751
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:79123
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:17 Jul 2017 19:29
Last Modified:10 Feb 2020 22:11

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